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HT10132

Bouin′s solution

histological fixative

Synonym(s):

Bouin’s fluid

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$98.50

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47
MDL number:
MFCD00146169

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shelf life

Expiry date on the label

composition

acetic acid, 5% , formaldehyde, 9% , picric acid, 0.9%

color

yellow

application(s)

hematology
histology

storage temp.

room temp

Quality Level

200

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This Item
HT1011283285721819
Bouin’s solution histological fixative

Sigma-Aldrich

HT10132

Bouin′s solution

Bouin’s solution histological fixative

Sigma-Aldrich

HT101128

Bouin′s solution

Wright Stain solution according to Wright

Sigma-Aldrich

32857

Wright Stain solution

Carbol-Fuchsin solution according to Kinyoun suitable for microscopy

Sigma-Aldrich

21819

Carbol-Fuchsin solution according to Kinyoun

color

yellow

color

yellow

color

-

color

red to very dark red

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

composition

acetic acid, 5% , picric acid, 0.9% , formaldehyde, 9%

composition

acetic acid, 5% , formaldehyde, 9% , picric acid, 0.9%

composition

-

composition

fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

shelf life

Expiry date on the label

shelf life

Expiry date on the label

shelf life

-

shelf life

-

General description

Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid.

Application

Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures.
Additionally, it has been used for the following purposes-
  • staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
  • fixation of rat ovaries for the observing follicle cells using microscopy
  • fixation of gonads of crab for morphological and histological analysis
Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Biochem/physiol Actions

Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation.

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000480539
0000480539
0000336639
0000336639
0000250408

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