Skip to Content
MilliporeSigma
All Photos(1)

Documents

HYSC020

Sigma-Aldrich

HyStem®-C Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution

Sign Into View Organizational & Contract Pricing

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

The HyStem® -C Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture. It is recommended for cultures, which require a minimal number of cell attachment sites or the addition of other extracellular matrix (ECM) proteins.

Application

HyStem® -C cell culture scaffold kit has been used for neural stem cell culture.

Features and Benefits

Customizable, well characterized and consistent, Hystem-C hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-C offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Synthetic - As a synthetic matrix, Hystem-C offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. Hystem-C contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), and Water.
  • Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-C hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.
Hystem-C enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

With Hystem-C, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-C has been successfully used to expand human embryonic stem cells, human mesenchymal stem cells, neural progenitor cells and hepatic progenitor cells.

Legal Information

Hystem is a registered trademark of BIOTIME, INC

Pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 5

1 of 5

Douglas O Sobel et al.
Islets, 12(4), 71-86 (2020-06-23)
Immortalized beta cells are an abundant source of insulin-producing cells. Although MIN-6 cells have similar characteristics as normal islets in vitro, the in vivo use of MIN-6 cells has not been fully described. This study characterizes in vivo mouse models
Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo
Basuodan R, et al.
Journal of Anatomy (2018)
Reem Basuodan et al.
Journal of anatomy, 233(2), 155-166 (2018-05-11)
Human neural stem cells (hNSC) derived from induced pluripotent stem cells can be differentiated into neurons that could be used for transplantation to repair brain injury. In this study we dispersed such hNSC in a three-dimensional artificial extracellular matrix (aECM)
Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo
Basuodan R, et al.
Journal of Anatomy (2018)

Articles

Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.

Heparin is distinct from heparan sulfate proteoglycans (HS) which is primarily produced by mast cells. While, HS is produced by all cell types. Know more about the difference between heparin and heparin sulfate proteoglycan and select the right component for your study.

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service