I0523

IL-2 mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): Interleukin-2, T-cell growth factor, mIL-2, IL-2
• MDL number: MFCD00164816
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Interleukin-2 from mouse, IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: mouse
• Quality Level: 200
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.2 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: 17.2 kDa
• packaging: pkg of 5X20 μg; pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white
• UniProt accession no.: P04351
• storage temp.: −20°C
• Gene Information: mouse ... Il2(16183)

Description

General description

IL-2 (interleukin-2) codes for a pro-inflammatory cytokine.[1] IL-2 is mostly expressed in T helper cells.[2]

Application

Interleukin-2 from mouse has been used to induce sepsis in rat model.[2] It has also been used in dendritic epidermal T cell culture.[3]

Biochem/physiol Actions

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lymphokine promotes long-term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of natural killer cells, induces γ-interferon and B cell growth factor secretion, and modulates the expression of the IL-2 receptor. IL-2 expression is known to reduce with age and results in influenza infection, neoplasms and lethal autoimmunity. IL-2 participates in T cell proliferation and differentiation and controls the homeostasis of T cell. As a part of immune response, IL-2 stimulates the action of T helper cell 1 and 2 along with endothelial white blood cells of skeletal muscles.[4][1] IL-2 regulation is controlled by c-rel protein.[4] IL-2 is a neurotrophic molecule and has special functions in cortical, hippocampal, septal, cerebellar and retinal neurons.[5]

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0.

Analysis Note

Activity was determined by dose-dependent stimulation of murine CTLL-2 cells. ED₅₀ : ≤ 0.2 ng/ml (corresponds to a specific activity of 5 × 10⁶ units/mg)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)