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I0634

Sigma-Aldrich

Ionomycin calcium salt

from Streptomyces conglobatus, ≥98% (HPLC), powder, Ca2+ ionophore

Synonym(s):

Ionomycin (calcium), SQ23377 calcium

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About This Item

Empirical Formula (Hill Notation):
C41H70CaO9
CAS Number:
Molecular Weight:
747.07
Beilstein/REAXYS Number:
1416163
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77

product name

Ionomycin calcium salt from Streptomyces conglobatus, powder, ≥98% (HPLC)

Quality Level

assay

≥98% (HPLC)

form

powder

storage condition

desiccated
protect from light

color

white to off-white

solubility

DMSO: 10 mg/mL
ethanol: soluble

antibiotic activity spectrum

Gram-positive bacteria

mode of action

cell membrane | interferes

storage temp.

2-8°C

SMILES string

C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O

InChI

1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

InChI key

WKRWUYKLUMMAKG-WYGBAUISSA-L

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General description

Calcium Ionomycin is a polyether antibiotic made by Streptomyces conglobatus. It can remove Ca2+ and divalent cations from an aqueous into an organic phase. Ionomycin enhances the influx of Ca2+ by triggering store-regulated cation entry rather than acting directly on the plasma membrane. It can induce apoptosis, potentially by activating a hidden enzyme that responds to calcium. Since calcium salt of ionomycin serves as a highly efficient transporter of Ca2+, it offers substantial benefits for studying the transport of Ca2+ across biological membranes. Ionomycin is employed to balance intracellular and extracellular calcium ion concentrations for in situ calibrations of fluorescent indicators.

Additionally, it is a narrow spectrum antibiotic effective against Gram-positive bacteria, obtained in pure form from the fermentation broths of Streptomyces conglobatus. It is unique amongst known polyether antibiotics due to its exceptional UV absorption maximum at 300 nm and its high affinity for calcium ions.

Application

Ionomycin calcium salt from Streptomyces conglobatus has been used as a media supplement to culture:

  • peripheral blood mononuclear cells (PBMCs) for immunologic assessment
  • oocytes for parthenogenetic activation
  • T cell subsets for the detection of intracellular cytokines, carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay, and antigen-specific suppressive function assay
  • in combination with phorbol12-myristate 13-acetate (PMA) to stimulate T-cell activation in various cells
  • as sperm capacitation medium supplement to study its impact on in vitro fertilization and increasing the efficiency of bovine embryo development
  • as a positive control for the detection of intracellular Ca2+ in adipose-derived stem cells (ASCs)(4)

Biochem/physiol Actions

Ca2+ ionophore that is more effective than A23187 as a mobile ion carrier for Ca2+; non-fluorescent; used to study Ca2+ transport across biological membranes; induces apoptotic degeneration of embryonic cortical neurons.
Ionomycin is an ionophore, widely used for validation and calibration of calcium analysis.

Preparation Note

Store ionomycin in powder form desiccated and protected from light at 2-8 °C. Under these conditions the product is stable for 3 years. Store stock solutions in ethanol or DMSO at -20 °C, protected from light. Under these conditions the stock solutions are stable for several months. For short term use (up to 6 weeks) the stock solutions maybe stored at 2-8 °C protected from light.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ionomycin, a new polyether antibiotic
LIU WC, et al.
The Journal of Antibiotics, 31, 815-819 (1978)
Comprehensive Biotechnology (2011)
Ionomycin enhances Ca2+ influx by stimulating store-regulated cation entry and not by a direct action at the plasma membrane
Morgan AJ and Jacob R
The Biochemical Journal, 300, 665-672 (1994)
Valproic acid promotes differentiation of adipose tissue-derived stem cells to neuronal cells selectively expressing functional N-type voltage-gated Ca2+ channels
Satoh A, et al.
Biochemical and Biophysical Research Communications, 589, 55-62 (2022)
The role of ionomycin in the improvement of in vitro fertilization and increasing the rate of bovine embryo development
Amran RA, et al.
Journal of Separation Science, 57(10), 1279-1284 (2023)

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