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I0634

Sigma-Aldrich

Ionomycin calcium salt from Streptomyces conglobatus

powder, ≥98% (HPLC)

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Synonym(s):
SQ23377 calcium
Empirical Formula (Hill Notation):
C41H70CaO9
CAS Number:
56092-82-1
Molecular Weight:
747.07
Beilstein:
1416163
MDL number:
MFCD00083634
PubChem Substance ID:
329815321
NACRES:
NA.77

Quality Level

300

Assay

≥98% (HPLC)

form

powder

storage condition

desiccated
protect from light

color

white to off-white

solubility

DMSO: 10 mg/mL
ethanol: soluble

antibiotic activity spectrum

Gram-positive bacteria

Mode of action

cell membrane | interferes

storage temp.

2-8°C

SMILES string

C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O

InChI

1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

InChI key

WKRWUYKLUMMAKG-WYGBAUISSA-L

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1 of 4

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L2913H0654F4425
Ionomycin calcium salt from Streptomyces conglobatus powder, ≥98% (HPLC)

Sigma-Aldrich

I0634

Ionomycin calcium salt from Streptomyces conglobatus

Leptomycin B solution from Streptomyces sp. ≥95% (HPLC), Supplied in methanol: water (7:3)

Sigma-Aldrich

L2913

Leptomycin B solution from Streptomyces sp.

Hygromycin B solution from Streptomyces hygroscopicus ≥60% (HPLC), 45-60 mg/mL in H2O

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H0654

Hygromycin B solution from Streptomyces hygroscopicus

Fostriecin sodium salt from Streptomyces pulveraceus ≥98% (HPLC)

Sigma-Aldrich

F4425

Fostriecin sodium salt from Streptomyces pulveraceus

form

powder

form

-

form

-

form

solid

storage condition

desiccated, protect from light

storage condition

protect from light

storage condition

-

storage condition

-

color

white to off-white

color

-

color

brown-yellow, light orange to dark orange, light yellow to dark yellow

color

-

solubility

DMSO: 10 mg/mL, ethanol: soluble

solubility

-

solubility

-

solubility

ethanol: soluble, methanol: soluble, water: soluble

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Application

Ionomycin calcium salt from Streptomyces conglobatus has been used as a media supplement to culture:
  • peripheral blood mononuclear cells (PBMCs) for immunologic assessment
  • oocytes for parthenogenetic activation
  • T cell subsets for the detection of intracellular cytokines, carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay and antigen-specific suppressive function assay

Biochem/physiol Actions

Ca2+ ionophore that is more effective than A23187 as a mobile ion carrier for Ca2+; non-fluorescent; used to study Ca2+ transport across biological membranes; induces apoptotic degeneration of embryonic cortical neurons.
Ionomycin is an ionophore, widely used for validation and calibration of calcium analysis.

Preparation Note

Store ionomycin in powder form desiccated and protected from light at 2-8 °C. Under these conditions the product is stable for 3 years. Store stock solutions in ethanol or DMSO at -20 °C, protected from light. Under these conditions the stock solutions are stable for several months. For short term use (up to 6 weeks) the stock solutions maybe stored at 2-8 °C protected from light.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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