I1011

Sigma-Aldrich

Anti-Human IgG (whole molecule) antibody produced in goat

whole antiserum

MDL number:
NACRES:
NA.46

Quality Level

biological source

goat

antibody form

whole antiserum

antibody product type

secondary antibodies

clone

polyclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:40,000
quantitative precipitin assay: 4.0 mg/mL

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.

Application

Anti-Human IgG (whole molecule) antibody has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • printing and processing of reverse phase protein microarrays
  • western blot
  • radial immunodiffusion

Biochem/physiol Actions

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.
IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Anti-Human IgG is supplied as a liquid containing 15 mM sodium azide as a preservative

Storage and Stability

For continuous use, store at2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

The role of Fc-receptors in the uptake and transport of therapeutic antibodies in the retinal pigment epithelium
Dithmer M, et al.
Experimental Eye Research, 145(1), 187-205 (2016)
Parmjeet S Randhawa et al.
Clinical and vaccine immunology : CVI, 13(9), 1057-1063 (2006-09-09)
Immunoglobulin G (IgG), IgA, and IgM antibodies were measured in serum samples from 71 organ donors, 81 kidney transplant recipients at transplantation, and 67 patients during the posttransplant period by using a virus-like particle-based enzyme-linked immunosorbent assay (ELISA). BK virus...
Pyruvate kinase M2 and the mitochondrial ATPase Inhibitory Factor 1 provide novel biomarkers of dermatomyositis: a metabolic link to oncogenesis
Santacatterina F, et al.
Journal of Translational Medicine, 15(1), 29-29 (2017)
Evaluation of the immune humoral response of Brazilian patients with Rubinstein-Taybi syndrome
Sugayama S M M, et al.
Brazilian journal of medical and biological research, 43(12), 1215-1224 (2010)
Fulvio Santacatterina et al.
Journal of translational medicine, 15(1), 29-29 (2017-02-12)
Metabolic alterations play a role in the development of inflammatory myopathies (IMs). Herein, we have investigated through a multiplex assay whether proteins of energy metabolism could provide biomarkers of IMs. A cohort of thirty-two muscle biopsies and forty plasma samples...

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