I1271

SAFC

LONG® R3 IGF-I human

recombinant, expressed in E. coli, Recombinant Analog, suitable for cell culture

Empirical Formula (Hill Notation):
C400H619N111O115S9
CAS Number:
Molecular Weight:
9111.45
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

recombinant

expressed in E. coli

assay

≥95% (HPLC)

form

powder

quality

endotoxin tested

application(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Application

LONG® R3 IGF-I human has been used:
  • for the in vitro direct stimulation of pancreatic islets
  • as a component of growth medium for retinal cell lines
  • to culture vascular smooth muscle cells (VSMCs)

LONG® R3 IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) that has been specifically engineered for the enhancement of cell culture performance. It is more biologically potent in vitro than either insulin or native IGF-I and has been shown to significantly increase recombinant protein production. It is ideal for both research and large-scale culture systems utilizing serum-free or low-level serum applications. LONG R3 IGF-I has been used with numerous cell types including CHO, vaccine-producing cell lines, fibroblasts and stem cells. All cell types that have a growth response to insulin in cell culture have the potential to respond to LONG R3 IGF-I. LONG R3 IGF-I provides equivalent or better performance to recombinant insulin depending on the cell line and clone.

LONG R3 IGF-I is produced in an Escherichia coli expression system without the use of animal-derived components.

Biochem/physiol Actions

A genetically engineered recombinant human analog of IGF-1.

Preparation Note

Lyophilized from 0.1M acetic acid

Legal Information

LONG is a registered trademark of Repligen Sweden AB

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do the sequences of Products I1146 and I1271 compare to that of native Insulin-like Growth Factor 1 (IGF-1)?

    The entire IGF-1 gene codes for 194 amino acids, but the mature form of the protein is 70 amino acids, consisting of amino acids 49-118.Product I1146 is R3 IGF-1, which is also 70 amino acids in length. The Glutamic acid (E) at position 3 has simply been replaced by an Arginine (R). So the sequence of Product I1146 is:GP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAProduct I1271 is the same as I1146, but with an additional thirteen amino acids "in front of" the normal N-terminus of the R3 IGF-1:MFPAM PLSSLFVNGP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSANote that the first 13 amino acids in the recombinant I1271 (MFPAMPLSSLFVN)are quite different from what would have been the preceding 13 amino acids in the immature IGF-1.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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