The specific buffer formulation used to prepare this product is considered proprietary. The buffer includes primarily Tris base, with sodium chloride and magnesium chloride (hexahydrate form), as well as a minimal amount of citric acid. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/420/993/i1643pis.pdf
I1643
Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)
powder, ≥500 units/mg protein (E1%/280)
Synonym(s):
Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 units | Available to ship TODAYfromMILWAUKEE | $138.00 | |
| 500 units | Available to ship TODAYfromMILWAUKEE | $372.00 | |
| 1000 units | Available to ship TODAYfromMILWAUKEE | $700.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-784-7
MDL number:
Specific activity:
≥500 units/mg protein (E1%/280)
$138.00
Available to ship TODAYDetails
form
powder
Quality Level
specific activity
≥500 units/mg protein (E1%/280)
mol wt
71 kDa
storage temp.
−20°C
General description
Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.
Application
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
It plays an important role in protein, RNA, and DNA synthesis.
Pyrophosphatase has been used:
Pyrophosphatase has been used:
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture
Physical form
Lyophilized powder containing 80-90% buffer salts
Other Notes
One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥500 units/mg protein (E1%/280) | specific activity ≥500 units/mg protein | specific activity ≥400 units/mg protein (biuret) | specific activity ≥50 units/mg protein |
| form powder | form ammonium sulfate suspension | form ammonium sulfate suspension | form lyophilized powder |
| mol wt 71 kDa | mol wt - | mol wt tetramer 119,500 Da±600 | mol wt 93 kDa |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Product Information Sheet
Laura Pedró-Rosa et al.
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Improved therapies for the treatment of Trypanosoma brucei, the etiological agent of the neglected tropical disease human African trypanosomiasis, are urgently needed. We targeted T. brucei methionyl-tRNA synthetase (MetRS), an aminoacyl-tRNA synthase (aaRS), which is considered an important drug target
Virginia P Ronchi et al.
The Journal of biological chemistry, 292(44), 18006-18023 (2017-09-20)
To understand the mechanism for assembly of Lys
Enzymatic chemiluminescence assay for inorganic pyrophosphate.
Vuokko Jansson et al.
Analytical biochemistry, 304(1), 135-137 (2002-04-24)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| I1643-100UN | 04061833847916 |
| I1643-500UN | 04061833847930 |
| I1643-1KU | 04061833847923 |
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We would like to make a "blank" without enzyme for the powder resuspended at 2 mg/mL in water. Could you please share the concentration of Tris, citric acid and magnesium chloride?
1 answer-
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I want to know what the endotoxin value is in this product(Pyrophosphatase, Sigma-Aldrich, I1643-1KU, LOT-SLCL2396).
1 answer-
This product is NOT tested for endotoxin. Please see the Certificate of Analysis for testing that is performed.
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