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I2411

Sigma-Aldrich

Isocitrate Dehydrogenase 1 (NADP+) human

recombinant, expressed in Sf9 cells, ≥90% (SDS-PAGE)

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Synonym(s):
IDH1, Isocitrate Dehydrogenase Cytoplasmic
CAS Number:
Enzyme Commission number:
NACRES:
NA.54

recombinant

expressed in Sf9 cells

Quality Level

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

47.6 kDa

concentration

≥0.30 mg/mL protein (Bradford)

NCBI accession no.

relevant disease(s)

cancer (lung)

shipped in

dry ice

storage temp.

−70°C

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This Item
I5036U6135U6510
Sigma-Aldrich

U6135

Ubiquitin-Specific Protease 8 human

Sigma-Aldrich

U6510

Ubiquitin-Specific Protease 5 human

concentration

≥0.30 mg/mL protein (Bradford)

concentration

-

concentration

≥0.10 mg/mL protein (Bradford)

concentration

≥0.20 mg/mL protein (Bradford), ≥51% (SDS-PAGE)

form

buffered aqueous glycerol solution

form

lyophilized powder

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

recombinant

expressed in Sf9 cells

recombinant

expressed in E. coli

recombinant

expressed in Sf9 cells

recombinant

expressed in Sf9 cells

mol wt

47.6 kDa

mol wt

46,658 Da

mol wt

128 kDa

mol wt

96 kDa

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

dry ice

General description

Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, full length [amino acids 1-414 (end)] with C-terminal FLAG-tag, expressed in Sf9 cells via a baculovirus expression system.

Application

Human Isocitrate Dehydrogenase (IDH1) is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. It may be useful as a biomarker for diagnosis and prognosis prediction of non-small cell lung cancer .

Biochem/physiol Actions

Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, is a full length amino acid with a C-terminal FLAG-tag and is expressed in Sf9 cells via a baculovirus expression system. IDH1 expression has been correlated with poor survival of non-small cell lung cancer. IDH1 promotes tumor growth .

Physical form

Solution in 45 mM Tris-HCl, 124 mM NaCl, 2.4 mM KCl, 90 μg/ml FLAG peptide, 3 mM DTT, and 10% glycerol at pH 8.0.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
Pack Size/Quantity

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Customers Also Viewed

Fengwei Tan et al.
Molecular & cellular proteomics : MCP, 11(2), M111-M111 (2011-11-09)
Lung cancer is the leading cause of cancer-related death in the world. To explore tumor biomarkers for clinical application, two-dimensional fluorescence difference gel electrophoresis and subsequent MALDI-TOF/TOF mass spectrometry were performed to identify proteins differentially expressed in 12 pairs of
Peter Paschka et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 28(22), 3636-3643 (2010-06-23)
To analyze the frequency and prognostic impact of isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) mutations in acute myeloid leukemia (AML). We studied 805 adults (age range, 16 to 60 years) with AML enrolled on German-Austrian AML Study
Yuejun Fu et al.
Biochemical and biophysical research communications, 397(2), 127-130 (2010-06-01)
Heterozygous mutations in either the R132 residue of isocitrate dehydrogenase I (IDH1) or the R172 residue of IDH2 in human gliomas were recently highlighted. Heterozygous mutations in the IDH1 occur in the majority of grade II and grade III gliomas
Lenny Dang et al.
Trends in molecular medicine, 16(9), 387-397 (2010-08-10)
The systematic sequencing of glioblastoma multiforme (GBM) genomes has identified the recurrent mutation of IDH1, a gene encoding NADP(+)-dependent isocitrate dehydrogenase 1 (IDH1) that catalyzes the oxidative decarboxylation of isocitrate yielding alpha-ketoglutarate (alpha-KG). Subsequent studies have confirmed recurrent IDH1 and
Varuna Sipayya et al.
Journal of cancer research and therapeutics, 8(4), 598-601 (2013-01-31)
Mutations in the gene encoding isocitrate dehydrogenase (IDH1) have been reported in gliomas. This study analyses a series of 184 glioma cases in a tissue microarray (TMA)-based approach to assess the frequency of R132H point mutations in formalin-fixed, paraffin-embedded tissue

Protocols

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Chromatograms

application for HPLC

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