I2511

Sigma-Aldrich

IgG from human serum

reagent grade, ≥95% (HPLC), buffered aqueous solution

Synonym(s):
Human IgG
MDL number:

Quality Level

grade

reagent grade

assay

≥95% (HPLC)

form

buffered aqueous solution

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Human IgG is purified from normal human serum by precipitation and gel filtration techniques.

Application

Purified human IgG has been used :-
  • as blocking antibody for flow cytometry
  • as negative control in chromatin immunoprecipitation assays
  • to precipitate IgG in human larynx epidermoid carcinoma cells by immunoprecipitation
  • as a standard in enzyme-linked immunosorbent assay

Biochem/physiol Actions

Immunoglobulin G (IgG) regulates the immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
In-Kyu Yoon et al.
The American journal of tropical medicine and hygiene, 72(6), 714-718 (2005-06-21)
Volunteers vaccinated with a candidate malaria vaccine containing merozoite surface protein 1(42) (MSP-1(42)) exhibit antibodies to MSP-1(42) that are measured by enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to make a human reference standard for MSP-1(42) antibody...
Wenjing Wang et al.
Soft matter, 12(30), 6451-6462 (2016-07-15)
Specifically designed bionanoparticles with a function-oriented protein-coating layer interact with self-prepared receptor surfaces as the counterpart. Based on surface plasmon resonance biosensing experiments, a model framework is validated to estimate the number of bonds formed between these bionanoparticles and the...
Kah Teong Soh et al.
Current protocols in cytometry, 75, 7-7 (2016-01-09)
Nucleic acid content can be quantified by flow cytometry through the use of intercalating compounds; however, measuring the presence of specific sequences has hitherto been difficult to achieve by this methodology. The primary obstacle to detecting discrete nucleic acid sequences...
Kentaro Matsuda et al.
The Journal of allergy and clinical immunology, 116(6), 1357-1363 (2005-12-13)
Mouse monoclonal IgE antibodies can promote the survival of mouse bone marrow-derived cultured mast cells and induce the cells to secrete mediators in the absence of known specific antigen. To determine whether human IgE, in the absence of known specific...
Andreia C Lino et al.
Immunity, 49(1), 120-133 (2018-07-15)
B lymphocytes can suppress immunity through interleukin (IL)-10 production in infectious, autoimmune, and malignant diseases. Here, we have identified a natural plasma cell subset that distinctively expresses the inhibitory receptor LAG-3 and mediates this function in vivo. These plasma cells also...

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