I2513

Sigma-Aldrich

Monoclonal Anti-Human IgG1 antibody produced in mouse

clone 8c/6-39, ascites fluid

Synonym(s):
Monoclonal Anti-Human IgG1 (Fc specific)
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

8c/6-39, monoclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:400

isotype

IgG2a

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 25, 6 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.
The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study.

Specificity

Human IgG consists of four subclasses (1-4) that can be recognized.

Application

Monoclonal Anti-Human IgG1 antibody produced in mouse has been used in:
  • direct hemagglutination(HA)
  • hemagglutination inhibition(HAI) assays
  • enzyme linked immunosorbent assay (ELISA)
  • immunofluorometric assay (IFMA)
  • immunofluorescence
  • cell-based assays for the detection of IgG subclasses of acetylcholine receptor (AChR) antibodies

Biochem/physiol Actions

Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes via Fc receptors (FcR). A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis.

Physical form

The antibody is provided as ascites fluid with 0.1% sodium azide as a preservative.

Storage and Stability

For continuous use, store at 0-5 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37-60

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Comparison of anti-acetylcholine receptor profiles between Chinese cases of adult- and juvenile-onset myasthenia gravis using cell-based assays
Yan C, et al.
Journal of Neuroimmunology, 349 (2020)
Human IgG isotypes and activating Fcgamma receptors in the interaction of Salmonella enterica serovar Typhimurium with phagocytic cells
Goh YS, et al.
Immunology, 133(1), 74-74 (2011)
Pro-inflammatory pattern of IgG1 Fc glycosylation in multiple sclerosis cerebrospinal fluid
Wuhrer,M, et al.
Journal of Neuroinflammation, 12 (2015)
Determination of IgG Subclasses and Avidity of Antithyroid Peroxidase Antibodies in Patients with Subclinical Hypothyroidism A Comparison with Patients with Overt Hypothyroidism
Silva LM, et al.
Hormone Research in p?diatrics, 59(3), 118-124 (2003)
Chong Yan et al.
Journal of neuroimmunology, 349, 577403-577403 (2020-09-30)
Juvenile-onset myasthenia gravis (JOMG) is a unique clinical subtype in China, featured by a higher prevalence of ocular myasthenia gravis (OMG), higher seronegativity of acetylcholine receptor (AChR) antibodies, and better prognosis than that in adult-onset myasthenia gravis (AOMG). We previously...
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
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