I2534

Sigma-Aldrich

Anti-Importin β antibody,Mouse monoclonal

clone 31H4, purified from hybridoma cell culture

Synonym(s):
Anti-p97, Anti-karyopherin β1, Anti-Imp β
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

31H4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~97 kDa

species reactivity

rat, mouse, human, bovine

application(s)

immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa total cell extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... KPNB1(3837)
mouse ... Kpnb1(16211)
rat ... Kpnb1(24917)

General description

Importin β comprises 19 tandemly repeated Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A), and the yeast kinase serine/threonine-protein kinase-TOR1 (HEAT) motifs and wraps around the importin-β (IBB). It also consists of an N-terminal RAN guanosine triphosphate (GTP) binding domain, a middle zone interacting with the nuclear pore, and an importin α binding domain at the C-terminus. The importin β gene is mapped to human chromosome location 17q21.32.
Monoclonal Anti-Importin b (mouse IgG1 isotype) is derived from the 31H4 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with importin. Importin β (Imp β), also referred to as karyopherin β1, is an import receptor that belongs to the family of nuclear transport receptors.

Specificity

Monoclonal Anti-Importin β recognizes human, bovine, rat, and mouse importin β.

Immunogen

importin β

Application

Anti-Importin β antibody,Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunofluorescent staining
  • coimmunoprecipitaion
  • western blotting
  • immunofluorescence
  • immunoprecipitation

Biochem/physiol Actions

Importin β (Impβ) forms a complex with importin α, which, in turn, binds the cargo protein via its NLS (nuclear localization signals). The cargo protein is transported by this ternary complex to the cytoplasmic face of the nuclear pore complex (NPC) via a direct interaction with specific nucleoporins. When the complex reaches the nuclear site of the nuclear pore complex (NPC), Ran-GTP binds to Impβ to form an Impβ/Ran-GTP complex and releases Impα and the cargo protein. The Impβ/Ran-GTP complex is then transported back to the cytoplasm, where the complex dissociates upon hydrolysis of GTP to GDP. Importin β is then ready to participate in new nuclear import cycle.
Importin β regulates cellular functions, such as mitotic spindle assembly and centrosome dynamics. It is implicated in the insertion of integral membrane proteins into the inner nuclear membrane. Importin β also functions as a chaperone for highly basic proteins in the cytoplasm.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Subcellular knockout of importin beta1 perturbs axonal retrograde signaling
Perry RBT, et al.
Neuron, 75, 294-305 (2012)
Anna Maria Copeland et al.
Journal of virology, 83(4), 1660-1668 (2008-12-17)
Replication of herpes simplex virus type 1 (HSV-1) involves a step in which a parental capsid docks onto a host nuclear pore complex (NPC). The viral genome then translocates through the nuclear pore into the nucleoplasm, where it is transcribed...
The effect of nanoparticle size and NLS density on nuclear targeting in cancer and normal cells; impaired nuclear import and aberrant nanoparticle intracellular trafficking in glioma
Tammam SN, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 253, 30-36 (2017)
Importin-beta-like nuclear transport receptors
Strom AC and Weis K
Genome Biology, 2(6) (2001)
Axoplasm isolation from peripheral nerve
Rishal I, et al.
Developmental neurobiology, 70(2), 126-133 (2010)

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