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I2911

Sigma-Aldrich

StableCell IMDM

With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Iscove’s Modified Dulbecco’s Medium, IMDM
MDL number:

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
FG0465I3390I6529
Sigma-Aldrich

Sigma-Aldrich

I2911

StableCell IMDM

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

Endotoxin, tested

impurities

Endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

components

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

components

stable glutamine: yes
NaHCO3: yes
glucose: yes
phenol red: yes

components

phenol red: yes
L-glutamine: no
glucose: yes

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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