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I3390

Sigma-Aldrich

Iscove′s Modified Dulbecco′s Medium

liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

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Synonym(s):
Iscoves MDM, IMDM
MDL number:
MFCD00217469
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
51471CI6529FG0465
Sigma-Aldrich

Sigma-Aldrich

I3390

Iscove′s Modified Dulbecco′s Medium

SAFC

SAFC

51471C

Iscove′s Modified Dulbecco′s Medium

SAFC

SAFC

I6529

Iscove′s Modified Dulbecco′s Medium

SAFC

SAFC

FG0465

Iscove′s Modified Dulbecco′s Medium

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

impurities

Endotoxin, tested

components

phenol red: yes
L-glutamine: no
glucose: yes

components

phenol red: 15.93 mg/L
NaHCO3: 3024 mg/L
L-glutamine: 582 mg/L
HEPES: 5958 mg/L
sodium pyruvate: 110 mg/L

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

components

stable glutamine: yes
NaHCO3: yes
glucose: yes
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Reconstitution

Supplement with 0.584 g/L L-glutamine

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supplement

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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