I3642

Sigma-Aldrich

Monoclonal Anti-Interleukin-1β antibody produced in mouse

clone 8516.311, purified immunoglobulin, lyophilized powder

Synonym(s):
Anti-IL-1β
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8516.311, monoclonal

form

lyophilized powder

species reactivity

human

application(s)

flow cytometry: suitable
immunohistochemistry: 8-25 μg/mL using human PBMC′s
neutralization: suitable
western blot: 1 μg/mL

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL1B(3553)

Specificity

The antibody will neutralize the biological activity of recombinant human IL-1β. It will not neutralize the activity of recombinant human IL-α, recombinant mouse IL-1α, or recombinant mouse IL-1β. When immobilized on a microplate, this antibody will capture both recombinant as well as natural human IL-1β. In immunoblotting, the antibody shows ~25% cross-reactivity with recombinant rat IL-1β and <5% cross-reactivity with recombinant mouse IL-1β and recombinant porcine IL-1β. Additionally, in immunoblotting, the antibody exhibits no cross-reactivity with recombinant mouse IL-α, recombinant human IL-1ra, recombinant mouse IL-1ra, or recombinant rat IL-1α.

Immunogen

purifed, E. coli-derived, recombinant human IL-1β.

Application

May used in immunoblotting, immunohistochemistry, ELISA, and neutralization assays

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Monoclonal Anti-Interleukin-1(IL-1) (mouse IgG1 isotype) is purified from a mouse hybridoma utilizing Protein A affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Certificate of Analysis
Certificate of Origin
Paul A Cordell et al.
Blood, 115(13), 2674-2681 (2010-01-21)
Factor XIII-A (FXIII-A) is present in the cytosol of platelets, megakaryocytes, monocytes, osteoblasts, and macrophages and may be released from cells by a nonclassical pathway. We observed that plasma FXIII-A levels were unchanged in thrombocytopenic mice (Bcl-x(Plt20/Plt20) and Mpl(-/-)), which...
Constanze Csaki et al.
Biochemical pharmacology, 75(3), 677-687 (2007-10-26)
The inflammatory process plays a pivotal role during the pathogenesis of osteoarthritis, dominated by catabolic processes initiated by pro-inflammatory cytokines such as IL-1beta. Resveratrol, a natural phytoalexin occurring in various fruits has previously been shown to exhibit anti-inflammatory properties in...
Ludmila Alekseeva et al.
BMC microbiology, 9, 33-33 (2009-02-12)
Aspergillus fumigatus, a saprophytic mould, is responsible for life-threatening, invasive pulmonary diseases in immunocompromised hosts. The role of the airway epithelium involves a complex interaction with the inhaled pathogen. Antimicrobial peptides with direct antifungal and chemotactic activities may boost antifungal...
Melanie Genoula et al.
Frontiers in immunology, 9, 459-459 (2018-03-30)
The ability of Mycobacterium tuberculosis (Mtb) to persist in its human host relies on numerous immune evasion strategies, such as the deregulation of the lipid metabolism leading to the formation of foamy macrophages (FM). Yet, the specific host factors leading...
Rosario García-Vicuña et al.
Arthritis and rheumatism, 50(12), 3866-3877 (2004-12-14)
To explore the potential involvement of the chemokine system in synoviocyte-mediated tissue destruction in rheumatoid arthritis (RA), we studied the expression profile of chemokine receptors and their function in the migration, proliferation, and matrix metalloproteinase (MMP) production of cultured fibroblast-like...

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