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I4131

Sigma-Aldrich

IgG from rat serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

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Synonym(s):
Rat IgG
MDL number:
NACRES:
NA.46

conjugate

unconjugated

Quality Level

grade

reagent grade

assay

≥95% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
I8015I5256I5381
vibrant-m

I4131

IgG from rat serum

vibrant-m

I8015

IgG from rat serum

vibrant-m

I5256

IgG from goat serum

vibrant-m

I5381

IgG from mouse serum

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

form

essentially salt-free, lyophilized powder

form

buffered aqueous solution

form

essentially salt-free, lyophilized powder

form

lyophilized powder

grade

reagent grade

grade

technical grade

grade

reagent grade

grade

reagent grade

assay

≥95% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

target post-translational modification

unmodified

target post-translational modification

-

target post-translational modification

unmodified

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids, Immunoglobins have two heavy (H) and two light (L) chains, linked by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4. IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Rat IgG is purified from pooled normal rat serum by fractionation and ion-exchange chromatography.

Application

IgG from rat serum has been used:
  • in immunolabeling
  • in flow cytometry
  • in enzyme linked immunosorbent assay
  • as a control in neutrophil depletion assay
Purified rat IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rat IgG was used as isotype control in flow cytometry and injected into mice to induce tolerance against rat IgG.

Biochem/physiol Actions

IgG antibody have enormous therapeutic potential and its fragment crystallizable (Fc) region contributes for the development of therapeutic antibody.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.

Protocols

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

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