I5020

Sigma-Aldrich

Anti-Interleukin 10 antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym(s):
Anti-IL-10
EC Number:
MDL number:
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

biological source

goat

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

application(s)

neutralization: suitable

conjugate

unconjugated

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL10(3586)

General description

Interleukin 10 is located on human chromosome 1q32.1.
Interleukin-10 (IL-10) is a pleotropic cytokine with anti-inflammatory and immunosuppressive functions. It is the key cytokine that regulates the host inflammatory responses to bacterial, fungal and viral pathogens. The production of IL-10 is mediated by signalling of IL-6, IL-12 and TGF-β. IL-10 is secreted by a variety of cells including T cells, B cells, dendritic cells, eosinophils, natural killer cells and mast cells. The effects of IL-10 are mediated by binding to the IL-10R, consisting of α and β subunits. The signalling through IL-10R activates Jak1, Tyk2 protein kinases and JAK/STAT3 pathways. IL-10 is involved in suppression of innate immunity to kill pathogens and inhibits the release of reactive oxygen and nitrogen species from activated macrophages. Regulation of IL-10 production is important in infections, inflammatory responses in autoimmune encephalomyelitis, endotoxic shock and chronic enterocolitis
Anti-interleukin-10 antibody is specific for human IL-10. The antibody shows ~7% cross reactivity with recombinant mouse IL-10 in ELISA and immunoblotting.

Specificity

The antibody neutralizes the biological activity of recombinant human IL-10, but not recombinant mouse IL-10.

Immunogen

recombinant human IL-10 expressed in Sf 21 insect cells.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Anti-interleukin 10 antibody produced in goat has been used to measure the anti-allodynic effect of IL-10 in the neuropathic pain rat model.
Anti-interleukin-10 antibody may be used for immunoblotting at a working concentration of 1 μg/ml. For ELISA a working concentration of 0.25 μg/ml is recommended. The antibody is suitable for neutralization reactions. This antibody has also been used for immunohistochemistry of cervicovaginal epithelial cells of rabbit

Biochem/physiol Actions

Interleukin 10 (IL10) helps to induce anti-tumor immune response. It controls tumor tolerance in non-small cell lung cancer. IL10 prevents the synthesis of pro-inflammatory cytokines by activating the JAK/STAT (janus kinase/signal transducers and activators of transcription) signalling pathway.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Preparation Note

Purified using protein G.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Toll-Like Receptors and cytokines as surrogate biomarkers for evaluating vaginal immune response following microbicide administration
Gupta SM et al
Mediators of Inflammation, doi:10-doi:10 (2008)
Genetic associations of the interleukin locus at 1q32. 1 with clinical outcomes of cutaneous melanoma.
Rendleman J, et al.
Journal of medical Genetics, 52(4), 231-239 (2015)
Chlamydia trachomatis inhibits the production of pro-inflammatory cytokines in human PBMCs through induction of IL-10.
Du K, et al.
Journal of Medical Microbiology, 67(2), 240-248 (2018)
Interleukin-10-regulated tumour tolerance in non-small cell lung cancer.
Vahl JM, et al.
British Journal of Cancer, 117(11), 1644-1644 (2017)
Yan Zhang et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 30(3), 113-122 (2010-03-02)
Apoptosis and the rapid clearance of apoptotic cells (ACs) by professional or nonprofessional phagocytes are normal and coordinated processes that ensure controlled cell growth and stress response with nonpathological outcomes. Uptake of ACs by phagocytes is thought to suppress autoimmune...

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