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I5032

Sigma-Aldrich

Anti-IKKγ/NEMO (C-Terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-IKKAP1
MDL number:

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~52 kDa

species reactivity

mouse, human, rat

technique(s)

western blot: 0.5-1 μg/mL

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... IKBKG(8517)
mouse ... Ikbkg(16151)
rat ... Ikbkg(309295)

General description

The gene IKKγ, also referred to as IKBKG (inhibitor of κ light polypeptide gene enhancer in B-cells, kinase γ) encodes the regulatory subunit of the inhibitor of κB kinase (IKK) complex, which also contains two catalytic subunits-IKK1/IKKα and IKK2/IKKβ. By phosphorylating IκB proteins, the IKK complex mediates the activation of NF (nuclear factor)-κB in response to a variety of stimuli. The gene is mapped to human chromosome Xq28.

Immunogen

synthetic peptide corresponding to amino acids 400-416 of human IKKγ.

Biochem/physiol Actions

The gene IKKγ, also referred to as NEMO (NF-κB essential modulator) interacts with IKKβ and is required for the activation of the IKK complex. Upon stimulation, IKK phosphorylates, ubiquitinates and degrades the NF-κB inhibitors, IκBα and IκBβ. This releases NF-κB, which in turn, enters the nucleus and activates target genes involved in immune and inflammatory responses. Mutations in this gene have been associated with incontinentia pigmenti, anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID), and other immunodeficiencies.

Features and Benefits

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Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Asma Smahi et al.
Human molecular genetics, 11(20), 2371-2375 (2002-09-28)
The transcription factor NF-kappaB regulates the expression of numerous genes controlling the immune and stress responses, inflammatory reaction, cell adhesion, and protection against apoptosis. Incontinentia pigmenti (IP) is the first genetic disorder to be ascribed to NF-kappaB dysfunction. IP is
E W Harhaj et al.
The Journal of biological chemistry, 274(33), 22911-22914 (1999-08-07)
The tax gene product of human T-cell leukemia virus type I induces activation of transcription factor NF-kappaB, which contributes to deregulated expression of various cellular genes. Tax expression triggers persistent phosphorylation and degradation of the NF-kappaB inhibitory proteins IkappaBalpha and
Tony T Huang et al.
Cell, 115(5), 565-576 (2003-12-04)
The transcription factor NF-kappaB is critical for setting the cellular sensitivities to apoptotic stimuli, including DNA damaging anticancer agents. Central to NF-kappaB signaling pathways is NEMO/IKKgamma, the regulatory subunit of the cytoplasmic IkappaB kinase (IKK) complex. While NF-kappaB activation by
C Makris et al.
Molecular cell, 5(6), 969-979 (2000-07-27)
IKK gamma/NEMO is the essential regulatory subunit of the I kappa B kinase (IKK), encoded by an X-linked gene in mice and humans. It is required for NF-kappa B activation and resistance to TNF-induced apoptosis. Female mice heterozygous for Ikk
D M Rothwarf et al.
Nature, 395(6699), 297-300 (1998-09-29)
Pro-inflammatory cytokines activate the transcription factor NF-kappaB by stimulating the activity of a protein kinase that phosphorylates IkappaB, an inhibitor of NF-kappaB, at sites that trigger its ubiquitination and degradation. This results in the nuclear translocation of freed NF-kappaB dimers

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