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I5036

Sigma-Aldrich

Isocitrate Dehydrogenase 1 (NADP+) human

recombinant, expressed in E. coli, lyophilized powder, ≥80 units/mg protein

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Synonym(s):
IDH1, Isocitrate Dehydrogenase Cytoplasmic
CAS Number:
Enzyme Commission number:
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥80 units/mg protein

mol wt

46,658 Da

storage temp.

−20°C

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This Item
I2411I1782SRE0024
assay

≥90% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

-

assay

-

form

lyophilized powder

form

buffered aqueous glycerol solution

form

solution

form

-

recombinant

expressed in E. coli

recombinant

expressed in Sf9 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

mol wt

46,658 Da

mol wt

47.6 kDa

mol wt

~55 kDa

mol wt

-

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

General description

Isocitrate dehydrogenase 1 (IDH1) gene is located on human chromosome 2q33. IDH1 is present in the cytoplasm and peroxisomes. It is expressed at high levels in the mammalian liver. IDH1 consists of a C-terminal tripeptide peroxisome, that targets the signal one sequence.

Biochem/physiol Actions

Isocitrate dehydrogenase 1 (IDH1) help in the oxidative carboxylation of isocitrate to α-ketoglutarate, that results in the formation of reduced nicotinamide adenine dinucleotide phosphate (NADPH). It participates in lipid metabolism and glucose sensing. Suppressing IDH1 in pancreatic islets affects the insulin secretion, induced by glucose and leads to increase in lactate production.

Unit Definition

One unit corresponds to the amount of enzyme which converts 1.0 μmol DL-isocitrate to α-ketoglutarate per minute at pH 7.4 and 37 °C (NADP as cofactor).

Physical form

Supplied as a lyophilized powder containing Tris-HCl pH 8.0, trehalose, ammonium sulfate and DTT

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Isocitrate dehydrogenase 1 codon 132 mutation is an important prognostic biomarker in gliomas
Sanson M, et al.
Journal of Clinical Oncology, 27(25), 4150-4154 (2009)
Mast cell tryptase and carboxypeptidase A expression in body fluid and gastrointestinal tract associated with drug-related fatal anaphylaxis
Guo X J, et al.
World Journal of Gastroenterology, 21(47), 13288-13288 (2015)
Chuan-Bao Zhang et al.
Journal of cancer research and clinical oncology, 140(1), 45-51 (2013-10-24)
Isocitrate dehydrogenase (IDH) gene mutation is one of the most exciting new advances in these years. It has been reported that IDH gene frequently altered in grade II and grade III gliomas. We aimed to identify the mutation frequency of
Anuhar Chaturvedi et al.
Blood, 122(16), 2877-2887 (2013-08-21)
Mutations in the metabolic enzymes isocitrate dehydrogenase 1 (IDH1) and 2 (IDH2) are frequently found in glioma, acute myeloid leukemia (AML), melanoma, thyroid cancer, and chondrosarcoma patients. Mutant IDH produces 2-hydroxyglutarate (2HG), which induces histone- and DNA-hypermethylation through inhibition of
Chunzhi Zhang et al.
Neuro-oncology, 15(9), 1114-1126 (2013-07-24)
Isocitrate dehydrogenase (IDH) enzymes have recently become a focal point for research aimed at understanding the biology of glioma. IDH1 and IDH2 are mutated in 50%-80% of astrocytomas, oligodendrogliomas, oligoastrocytomas, and secondary glioblastomas but are seldom mutated in primary glioblastomas.

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