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I5381

Sigma-Aldrich

IgG from mouse serum

reagent grade, ≥95% (SDS-PAGE), lyophilized powder

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Synonym(s):
Mouse IgG
MDL number:

conjugate

unconjugated

Quality Level

grade

reagent grade

assay

≥95% (SDS-PAGE)

form

lyophilized powder

composition

Protein, ≥80%

storage temp.

2-8°C

target post-translational modification

unmodified

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1 of 4

This Item
I5006I4131I5506
vibrant-m

I5381

IgG from mouse serum

vibrant-m

I5006

IgG from rabbit serum

vibrant-m

I4131

IgG from rat serum

vibrant-m

I5506

IgG from bovine serum

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

grade

reagent grade

grade

reagent grade

grade

reagent grade

grade

reagent grade

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

composition

Protein, ≥80%

composition

-

composition

-

composition

-

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.

Application

Purified mouse IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors.

Physical form

Lyophilized from 0.01 M phosphate buffer, pH 7.2, with 0.015 M NaCl

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Fluorescent Biorecognition of Gold Nanoparticle-IgG Conjugates Self-Assembled on E-Beam Patterns
Powell T and Yoon JY
Biotechnol. Progress, 22, 106-110 (2006)
Lonnie J Lucas et al.
Biosensors & bioelectronics, 23(5), 675-681 (2007-09-18)
Double detection of microsphere light scattering and quantum dot emission was demonstrated for lab-on-a-chip immunoassay without using stationary support. We conjugated quantum dots (QDs) onto microspheres to enable multiplex assays as well as to enhance the limit of detection (LOD).
C Rebouissou et al.
Blood, 91(12), 4727-4737 (1998-06-17)
Agonist antihuman gp130 transducer monoclonal antibodies (MoAbs) were used in SCID mice to grow myeloma cells whose survival and proliferation is dependent on gp130 transducer activation. The agonist anti-gp130 MoAbs neither bound to murine gp130 nor activated murine cells and
Paurvi Shinde et al.
Journal of leukocyte biology, 102(1), 57-69 (2017-04-23)
LPS is a powerful adjuvant, and although LPS-mediated TLR4 signaling has been exquisitely delineated, the in vivo mechanism of how TLR4 responses impact T cell priming is far less clear. Besides costimulation, TNF and type 1 IFN are dominant cytokines
Panojot Bifsha et al.
Molecular neurobiology, 54(7), 4921-4935 (2016-08-16)
Dysfunction of midbrain dopaminergic (mDA) neurons is involved in Parkinson's disease (PD) and neuropsychiatric disorders. Pitx3 is expressed in mDA neuron subsets of the substantia nigra compacta (SNc) and of the ventral tegmental area (VTA) that are degeneration-sensitive in PD.

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