I5885

Sigma-Aldrich

Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse

clone GG-5, ascites fluid

Synonym(s):
Monoclonal Anti-Human IgG (γ-chain specific)
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

GG-5, monoclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:20,000

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

Binds human IgG; does not bind other human Igs.The antibody is specific for a determinant on the heavy chain of human IgG. This antibody will form antigen-antibody complexes in the liquid phase in the presence of 3% PEG 6000.
Monoclonal Anti-Human IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG.

Application

Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).
Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).

Biochem/physiol Actions

Immunoglobulin G (IgG) mainly helps in immune defense. IgG participates in hypersensitivity type II and type III.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37-60

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Charles Pilette et al.
Immunology and cell biology, 88(4), 486-493 (2010-01-20)
As previously reported by others for immunoglobulin (Ig)G, we observed that IgA can induce interleukin (IL)-10 expression in human monocytes. In this study, we explored the molecular mechanisms of IL-10 induction by IgA in monocytes and monocyte-derived dendritic cells (MD-DCs)....
The Immunoglobulins: Structure and Function (1998)
Utility of the P 19 suppressor of gene-silencing protein for production of therapeutic antibodies in N icotiana expression hosts
Garabagi F, et al.
Plant Biotechnology Journal, 10(9), 1118-1128 (2012)
Molecular Genetics of Immunoglobulin (1987)
Freydoun Garabagi et al.
Plant biotechnology journal, 10(9), 1118-1128 (2012-09-19)
To study how the P19 suppressor of gene-silencing protein can be used effectively for the production of therapeutic glycoproteins, the following factors were examined: the genetic elements used for expressing recombinant proteins; the effect of different P19 concentrations; compatibility of...
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
Read More

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