I6135

Sigma-Aldrich

Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse

clone GG-6, ascites fluid

MDL number:
NACRES:
NA.46

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GG-6, monoclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:20,000

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

General description

Monoclonal Anti-Human IgG (Fab Specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) is found in the blood. It is present in the human serum, secreted by the B cells. IgG has the lengthiest serum half-life compared to other immunoglobulins. Heavy and light chains of IgG are interconnected by disulfide bonds.
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. Monoclonal Anti-Human IgG (Fab specific) antibody is specific for the Fab fragment of human IgG. The antibody does not react with the Fc portion of human IgG.

Specificity

Monoclonal Anti-Human IgG (Fab Specific) is immunospecific for human IgG. The antibody reacts with the Fab fragment of IgG.

Immunogen

Purified human IgG

Application

Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Maternal IgG passes through the placenta to the fetus and confers immune defense of the neonate against infections. IgG is implicated in antibody-dependent cell-mediated cytotoxicity (ADCC) and also nullifies virus particles and toxins. Papain digestion of IgG generates fragment antigen-binding (Fab). Pepsin digestion of IgG produces fragment crystallizable (fc) fragment and a single dimeric F(ab)2.
These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide (see MSDS) as a preservative.

Storage and Stability

For continuous use, store at 0-5 °C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Ian P Hurley et al.
Forensic science international, 190(1-3), 91-97 (2009-07-07)
The detection and confirmation of bloodstains as being human in origin is important in crime scene investigations. There are a number of blood detection methods currently available. The aim of this work was to develop an assay capable of detecting...
Mario R Romero et al.
Acta odontologica latinoamericana : AOL, 24(2), 176-182 (2011-12-15)
The aim of this study was to measure the level of immunoglobulin A, G and M in saliva of 3- to 12-year-old children, both healthy and diagnosed with gingivitis. A sample of 177 children was selected, of whom 24 were...
D M Kennedy et al.
Clinical and experimental immunology, 98(2), 245-251 (1994-11-01)
As elevated levels of glycated IgG have been detected in the plasma of patients with diabetes mellitus, a disease associated with increased susceptibility to infection, we have investigated whether glycation of MoAbs affects the kinetics and/or affinity of antigen binding....
Maria L Balmer et al.
Science translational medicine, 6(237), 237ra66-237ra66 (2014-05-23)
A prerequisite for establishment of mutualism between the host and the microbial community that inhabits the large intestine is the stringent mucosal compartmentalization of microorganisms. Microbe-loaded dendritic cells trafficking through lymphatics are arrested at the mesenteric lymph nodes, which constitute...
Ryuta Uraki et al.
Vaccine, 32(41), 5295-5300 (2014-08-12)
Because vaccination is an effective means to protect humans from influenza viruses, extensive efforts have been made to develop not only new vaccines, but also for new adjuvants to enhance the efficacy of existing inactivated vaccines. Here, we examined the...

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