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I6135

Sigma-Aldrich

Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse

clone GG-6, ascites fluid

MDL number:
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GG-6, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4[1][2]. Monoclonal Anti-Human IgG (Fab specific) antibody is specific for the Fab fragment of human IgG. The antibody does not react with the Fc portion of human IgG.
Monoclonal Anti-Human IgG (Fab Specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) is found in the blood.[3] It is present in the human serum, secreted by the B cells.[4][3] IgG has the lengthiest serum half-life compared to other immunoglobulins.[3] Heavy and light chains of IgG are interconnected by disulfide bonds.[4]

Specificity

Monoclonal Anti-Human IgG (Fab Specific) is immunospecific for human IgG. The antibody reacts with the Fab fragment of IgG.

Immunogen

Purified human IgG

Application

Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA).[5][6][7]

Biochem/physiol Actions

Maternal IgG passes through the placenta to the fetus and confers immune defense of the neonate against infections.[8] IgG is implicated in antibody-dependent cell-mediated cytotoxicity (ADCC)[4] and also nullifies virus particles and toxins.[4] Papain digestion of IgG generates fragment antigen-binding (Fab). Pepsin digestion of IgG produces fragment crystallizable (fc) fragment and a single dimeric F(ab)2.[3]
These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.[1][2]

Physical form

The product is provided as ascites fluid with 0.1% sodium azide (see MSDS) as a preservative.

Storage and Stability

For continuous use, store at 0-5 °C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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