Monoclonal Anti-Human IgG (Fab Specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) is found in the blood. It is present in the human serum, secreted by the B cells. IgG has the lengthiest serum half-life compared to other immunoglobulins. Heavy and light chains of IgG are interconnected by disulfide bonds.
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. Monoclonal Anti-Human IgG (Fab specific) antibody is specific for the Fab fragment of human IgG. The antibody does not react with the Fc portion of human IgG.
Monoclonal Anti-Human IgG (Fab Specific) is immunospecific for human IgG. The antibody reacts with the Fab fragment of IgG.
Purified human IgG
Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA).
Maternal IgG passes through the placenta to the fetus and confers immune defense of the neonate against infections. IgG is implicated in antibody-dependent cell-mediated cytotoxicity (ADCC) and also nullifies virus particles and toxins. Papain digestion of IgG generates fragment antigen-binding (Fab). Pepsin digestion of IgG produces fragment crystallizable (fc) fragment and a single dimeric F(ab)2.
These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
The product is provided as ascites fluid with 0.1% sodium azide (see MSDS) as a preservative.
Storage and Stability
For continuous use, store at 0-5 °C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
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