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I6135

Sigma-Aldrich

Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse

clone GG-6, ascites fluid

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MDL number:
NACRES:
NA.46

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GG-6, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
I5385I9388SAB4200682
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

GG-6, monoclonal

clone

SG-16, monoclonal

clone

HP-6001, monoclonal

clone

GG-7, monoclonal

technique(s)

indirect ELISA: 1:20,000

technique(s)

indirect ELISA: 1:5,000

technique(s)

indirect ELISA: 1:3,000

technique(s)

ELISA: suitable, immunofluorescence: suitable, indirect ELISA: 0.25-0.5 μg/mL using 5μg/mL human IgG for coating.

isotype

IgG1

isotype

IgG1

isotype

IgG2b

isotype

IgG1

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. Monoclonal Anti-Human IgG (Fab specific) antibody is specific for the Fab fragment of human IgG. The antibody does not react with the Fc portion of human IgG.
Monoclonal Anti-Human IgG (Fab Specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) is found in the blood. It is present in the human serum, secreted by the B cells. IgG has the lengthiest serum half-life compared to other immunoglobulins. Heavy and light chains of IgG are interconnected by disulfide bonds.

Specificity

Monoclonal Anti-Human IgG (Fab Specific) is immunospecific for human IgG. The antibody reacts with the Fab fragment of IgG.

Immunogen

Purified human IgG

Application

Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Maternal IgG passes through the placenta to the fetus and confers immune defense of the neonate against infections. IgG is implicated in antibody-dependent cell-mediated cytotoxicity (ADCC) and also nullifies virus particles and toxins. Papain digestion of IgG generates fragment antigen-binding (Fab). Pepsin digestion of IgG produces fragment crystallizable (fc) fragment and a single dimeric F(ab)2.
These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide (see MSDS) as a preservative.

Storage and Stability

For continuous use, store at 0-5 °C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Mario R Romero et al.
Acta odontologica latinoamericana : AOL, 24(2), 176-182 (2011-12-15)
The aim of this study was to measure the level of immunoglobulin A, G and M in saliva of 3- to 12-year-old children, both healthy and diagnosed with gingivitis. A sample of 177 children was selected, of whom 24 were
Ian P Hurley et al.
Forensic science international, 190(1-3), 91-97 (2009-07-07)
The detection and confirmation of bloodstains as being human in origin is important in crime scene investigations. There are a number of blood detection methods currently available. The aim of this work was to develop an assay capable of detecting
Udayan Ray et al.
Journal of thrombosis and thrombolysis, 33(3), 218-229 (2012-01-13)
Insulin inhibits platelet aggregation through nitric oxide synthesis by stimulating platelet insulin activated nitric oxide synthase. Impaired platelet insulin activated nitric oxide synthase in acute myocardial infarction (AMI) patients had been reported and thus our aim was to identify and
Manuel Montesinos-Rongen et al.
Journal of immunology (Baltimore, Md. : 1950), 195(3), 1312-1319 (2015-06-28)
Primary lymphoma of the CNS (PCNSL) is a diffuse large B cell lymphoma confined to the CNS. To elucidate its peculiar organ tropism, we generated recombinant Abs (recAbs) identical to the BCR of 23 PCNSLs from immunocompetent patients. Although none
F Cataldo et al.
Minerva pediatrica, 42(12), 509-514 (1990-12-01)
IgG are the most common isotype of Ig and include four subclasses which differ from one another in the following ways: their initial amino acid sequence, their physical and chemical properties and the different serum concentrations reached with age. Every

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