I6510

Sigma-Aldrich

Monoclonal Anti-Human IgE antibody produced in mouse

clone GE-1, ascites fluid

Synonym(s):
Monoclonal Anti-Human IgE
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

GE-1, monoclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:5,000

isotype

IgG2b

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin E (IgE) antibodies are tetramers with two light chains (κ or λ) and two ε-heavy connected by numerous intrachain disulfide bonds. IgE exists in two isoforms, one is associated with the B cell receptor and the other being secreted by plasma cell. It has a short life span in plasma when compared to receptor bounded IgE.
Binds human IgE; does not bind human IgG, IgA or IgM.

Specificity

Monoclonal Anti-Human IgE is immunospecific for human IgE. No cross-reactivity with human IgG, IgM or purified light chains is observed.

Application

To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Biochem/physiol Actions

Immunoglobulin E (IgE) is implicated in acute allergic reactions and chronic inflammatory allergic diseases, such as allergic asthma and chronic urticaria (CU). Receptor bounded IgE aids antigen uptake and modulates the differentiation and proliferation of B cells. It confers immunity against helminthic parasites. IgE is also involved in facilitating type I hypersensitivity reactions. It regulates mast cell homeostasis and also exhibits immunomodulatory effects.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Analysis Note

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37-60

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Wondossen Sime et al.
Journal of immunology (Baltimore, Md. : 1950), 183(7), 4657-4665 (2009-09-16)
Mast cells (MCs) are multifunctional effectors of the immune system that are distributed in many tissues, often in close association with the basement membrane of blood vessels, epithelium and nerves. Laminins (LMs), a family of large alphabetagamma heterotrimeric proteins, are...
P Demoly et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 32(7), 1071-1076 (2002-07-09)
Cupressaceae pollen allergy is a world-wide pollinosis but immunotherapy has rarely been tested. Immunotherapy is usually allergen-specific but new forms may be targeted towards IgE. A randomized, double-blind, placebo-controlled trial was carried out to assess the efficacy of a vaccine...
X Su et al.
Analytical biochemistry, 273(1), 66-72 (1999-08-24)
This work presents a piezoelectric (Pz) immunosensor for the quantification of total human IgE in serum samples. The anti-human IgE is deposited on the surface of the 10 M Hz AT-cut gold coated crystal resonator by self-assembled technique, and serves...
Marjoke M Verweij et al.
Cytometry. Part B, Clinical cytometry, 78(5), 302-307 (2010-06-10)
P38 mitogen-activated protein kinase (MAPK) is known to govern IgE-mediated basophil activation. Intracellular phosphorylated p38 MAPK (Pp38 MAPK) in IgE-activated basophils can be quantified flow cytometrically. To study whether Pp38 MAPK constitutes a potential novel read-out for flow-assisted diagnosis of...
Lawren C Wu et al.
Nature reviews. Immunology, 14(4), 247-259 (2014-03-15)
IgE not only provides protective immunity against helminth parasites but can also mediate the type I hypersensitivity reactions that contribute to the pathogenesis of allergic diseases such as asthma, allergic rhinitis and atopic dermatitis. Despite the importance of IgE in...

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