MilliporeSigma
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I6510

Sigma-Aldrich

Monoclonal Anti-Human IgE antibody produced in mouse

clone GE-1, ascites fluid

Synonym(s):
Monoclonal Anti-Human IgE
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

GE-1, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:5,000

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

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General description

Binds human IgE; does not bind human IgG, IgA or IgM.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin E (IgE) antibodies are tetramers with two light chains (κ or λ) and two ε-heavy connected by numerous intrachain disulfide bonds.[1] IgE exists in two isoforms, one is associated with the B cell receptor and the other being secreted by plasma cell.[2] It has a short life span in plasma when compared to receptor bounded IgE.[3]

Specificity

Monoclonal Anti-Human IgE is immunospecific for human IgE. No cross-reactivity with human IgG, IgM or purified light chains is observed.

Application

To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Biochem/physiol Actions

Immunoglobulin E (IgE) is implicated in acute allergic reactions and chronic inflammatory allergic diseases, such as allergic asthma and chronic urticaria (CU).[3] Receptor bounded IgE aids antigen uptake and modulates the differentiation and proliferation of B cells.[2] It confers immunity against helminthic parasites. IgE is also involved in facilitating type I hypersensitivity reactions.[2] It regulates mast cell homeostasis and also exhibits immunomodulatory effects.[1]

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Analysis Note

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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