I7260

Sigma-Aldrich

Monoclonal Anti-Human IgG3 antibody produced in mouse

clone HP-6050, ascites fluid

Synonym(s):
Monoclonal Anti-Human IgG3
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6050, monoclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:10,000

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

Human IgG consists of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physicochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies.
Monoclonal Anti-Human IgG3 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Monoclonal Anti-Human IgG3 (I7260) is specific for the IgG3 subclass and does no react with IgG1, IgG2, and IgG4 in an ELISA. The IUIS/WHO12 study singled out this monoclonal antibody as one of the most widely applicable IgG3 specific monoclonal antibodies.
The antibody is specific for human IgG3 and is non-reactive with other IgG subclasses. This clone has been recommended by the IUIS/WHO study for its specificity and reactivity in a wide range of assay protocols and techniques.

Immunogen

Purified human IgG3 myeloma proteins covalently coupled to poly aminostyrene (PAS) microbeads were used as the immunogen.
Human IgG3

Application

Anti-Human IgG3 may be used for the identification of the IgG3 subclass by various immunoassays including enzyme-linked immunosorbent assay (ELISA), imprint immunofixation (IIF), immunofluorometric assay (IFMA), hemagglutination (HA), hemagglutination inhibition (HAI), particle counting immunoassay (PACIA), and detection of cytoplasmic IgG.

Biochem/physiol Actions

IgG3 exhibits protective action against wide range of intracellular bacteria, parasites and viruses. IgG3 antibodies potentially mediate various functions including, enhanced antibody-dependent cellular cytotoxicity (ADCC), opsonophagocytosis, complement activation and neutralization, compared with other IgG subclasses. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Ratio of serum IgG3 to total IgG concentration and goiter size are independent factors in intractability of Graves' disease
Santoh T, et al.
Endocrine Journal, 887-894 (2007)
Nicolas Dauby et al.
PLoS neglected tropical diseases, 3(12), e571-e571 (2009-12-31)
We previously showed that newborns congenitally infected with Trypanosoma cruzi (M+B+) display a strong type 1 parasite-specific T cell immune response, whereas uninfected newborns from T. cruzi-infected mothers (M+B-) are prone to produce higher levels of proinflammatory cytokines than control...
Anna Babakhanyan et al.
Open forum infectious diseases, 3(2), ofw092-ofw092 (2017-05-11)
Background.  Human immunodeficiency virus (HIV) infection reduces placental transfer of antibodies from mother to the fetus for many antigens;...
Maternal human immunodeficiency virus-associated hypergammaglobulinemia reduces transplacental transfer of immunoglobulin G to Plasmodium falciparum antigens in Cameroonian neonates
Babakhanyan A, et al.
Open forum infectious diseases, 3(2), ofw092-ofw092 (2016)
Induction of IgG3 secretion by interferon gamma: a model for T cell-independent class switching in response to T cell-independent type 2 antigens
Snapper CM, et al.
The Journal of Experimental Medicine, 175(5), 1367-1371 (1992)
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
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