I7385

Sigma-Aldrich

Monoclonal Anti-Human IgG4 antibody produced in mouse

clone HP-6025, ascites fluid

Synonym(s):
Monoclonal Anti-Human IgG4
NACRES:
NA.46
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6025, monoclonal

contains

15 mM sodium azide

application(s)

immunofluorescence: suitable
indirect ELISA: 1:5,000

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines. Unlike other immunoglobulin G is rare and constitutes to only 3-4 percent of the total IgG in the serum.

Specificity

Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.

Application

The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.
Monoclonal Anti-HuIgG4 man antibody produced in mouse has been used in ELISA and immunofluorescence.

Biochem/physiol Actions

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differing from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4. Increased levels of IgG4 is observed in atopic dermatitis, parasitic diseases, pemphigus vulgaris and pemphigus foliaceus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37-60

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

High serum IgG4 concentrations in patients with sclerosing pancreatitis.
Hamano H, et al.
The New England Journal of Medicine, 344(10), 732-738 (2001)
Francois Romagné et al.
Blood, 114(13), 2667-2677 (2009-06-26)
Inhibitory-cell killer immunoglobulin-like receptors (KIR) negatively regulate natural killer (NK) cell-mediated killing of HLA class I-expressing tumors. Lack of KIR-HLA class I interactions has been associated with potent NK-mediated antitumor efficacy and increased survival in acute myeloid leukemia (AML) patients...
Preclinical characterization of 1-7F9, a novel human anti?KIR receptor therapeutic antibody that augments natural killer?mediated killing of tumor cells.
Romagne F, et al.
Blood, 114(13), 2667-2677 (2009)
Charge and size selectivity of proteinuria in children with idiopathic nephrotic syndrome.
Taylor G M, et al.
Pediatric Nephrology (Berlin), 11(4), 404-410 (1997)
L M Silva et al.
Hormone research, 59(3), 118-124 (2003-03-15)
To evaluate the immunoglobulin G subclasses of anti-TPO and antibody avidity in patients with subclinical hypothyroidism (sH), overt hypothyroidism (H) and a control group (C). According to the TSH, fT4 and anti-TPO antibody levels, appraised by immunometric assays, 95 female...

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