I8135

IgM from bovine serum

reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution

• Synonym(s): Bovine IgM
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00163154
• Conjugate: unconjugated
• Species reactivity: -
• Application: —
• Citations: 10

Properties

• conjugate: unconjugated
• grade: reagent grade
• assay: ≥95% (SDS-PAGE or HPLC)
• form: buffered aqueous solution
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Quality Level: 300

Description

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens[1]
IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.

IgM is a highly conserved antibody in vertebrates and is expressed early during immune response.[2] It exists as a pentamer and is secreted by peritoneal B cells.[3]

Application

IgM from bovine serum has been used for screening the lipopolysaccharide levels in Limulus amoebocyte lysate (LAL) assay.[4] IgM has been used in flow cytometry assay for detecting FcγRIV expression and immunoglobulin binding.[5]

Purified bovine IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.

Biochem/physiol Actions

IgM plays a key role in engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections.[3] IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.[6]

Physical form

Solution in 0.05 M Tris-HCl, 0.15 M sodium chloride and 0.01 M glycine, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable