I8135

Sigma-Aldrich

IgM from bovine serum

reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution

Synonym(s):
Bovine IgM
MDL number:

Quality Level

grade

reagent grade

assay

≥95% (SDS-PAGE or HPLC)

form

buffered aqueous solution

conjugate

unconjugated

shipped in

wet ice

storage temp.

2-8°C

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells.

Application

IgM from bovine serum has been used for screening the lipopolysaccharide levels in Limulus amoebocyte lysate (LAL) assay. IgM has been used in flow cytometry assay for detecting FcγRIV expression and immunoglobulin binding.
Purified bovine IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.

Biochem/physiol Actions

IgM plays a key role in engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Physical form

Solution in 0.05 M Tris-HCl, 0.15 M sodium chloride and 0.01 M glycine, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

IgE b immune complexes activate macrophages through FcgammaRIV binding
Hirano M, et al.
Nature Immunology, 8(7), 762-762 (2007)
Lipopolysaccharide is a frequent and significant contaminant in microglia-activating factors
Weinstein JR, et al.
Glia, 56(1), 16-26 (2008)
Emerging functions of natural IgM and its Fc receptor FCMR in immune homeostasis
Wang H, et al.
Frontiers in Immunology, 7, 99-99 (2016)
Protective roles of natural IgM antibodies
Gronwall C, et al.
Frontiers in Immunology, 3, 66-66 (2012)
The importance of natural IgM: scavenger, protector and regulator
Ehrenstein MR and Notley, Clare A
Nature Reviews: Immunology, 10(11), 778-778 (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service