The IgM antibody with product code I8260 is not tested for molecular weight, as it consists of two heavy and two light chains. For the unmodified human IgM subunit chains, one publication reports the following molecular weights: m chain = 75 kDa, k chain = 22 kDa. Refer to the publication "Habeeb, A.F.S.A., et al., Studies of Maleylated Chains of Human IgM. J. Immunol., 105, 846-855 (1970)" for more information. Additionally, the monomer of the IgA product with code I4036 is expected to have a molecular weight of 160 kDa.
I8260
IgM from human serum
reagent grade, ~95% (HPLC), buffered aqueous solution
Synonym(s):
Human IgM
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About This Item
Conjugate:
unconjugated
Application:
—
Citations:
76
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conjugate
unconjugated
grade
reagent grade
assay
~95% (HPLC)
form
buffered aqueous solution
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
1 of 4
This Item | I8135 | I8265 | I8765 |
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| grade reagent grade | grade reagent grade | grade technical grade | grade technical grade |
| assay ~95% (HPLC) | assay ≥95% (SDS-PAGE or HPLC) | assay ≥80% (SDS-PAGE) | assay ≥80% (SDS-PAGE) |
Application
Purified human IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. IgM from human serum was used as standard in ELISA (100 μg/ml)[1] and dot blot assay.[2] Antibody concentration range of 200 μg/ml to 1 mg/ml was used in IgM binding assays.[3][4]
IgM from human serum has been used
IgM from human serum has been used
Biochem/physiol Actions
Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.[9]
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five or six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.[9]
IgM antibodies are present as pentamers in the serum and are produced in response to antigens.[10]
IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens.[10]
IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures.
Physical form
Solution in 0.05 M Tris-HCl, 0.2 M sodium chloride, pH 8.0, containing 15 mM sodium azide.
Preparation Note
Store at 2-8 °C. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Isabelle Leduc et al.
Infection and immunity, 77(2), 657-666 (2008-11-19)
Resisting the bactericidal activity of naturally occurring antibodies and complement of normal human serum is an important element in the evasion of innate immunity by bacteria. In the gram-negative mucosal pathogen Haemophilus ducreyi, serum resistance is mediated primarily by the
Localization of the domains of the Haemophilus ducreyi trimeric autotransporter DsrA involved in serum resistance and binding to the extracellular matrix proteins fibronectin and vitronectin.
Leduc I, et al.
Infection and Immunity, 77(2), 657-666 (2009)
Selection of mimotopes of the cell surface adhesion molecule Mel-CAM from a random pVIII-28aa phage peptide library.
Hafner C, et al.
The Journal of Investigative Dermatology, 119(4), 865-869 (2002)
L Rydberg et al.
Xenotransplantation, 5(2), 105-110 (1998-12-16)
A quantitative ELISA technique for determination of human anti-pig xenoantibody number in serum samples has been established using pig lymphocytes and pig/rabbit erythrocytes as target cells and a pool of serum from human blood group AB donors. The number of
Apolline Salama et al.
Transplantation, 101(10), 2501-2507 (2017-02-16)
Polyclonal antihuman thymocyte rabbit IgGs (antithymocyte globulin [ATG]) are popular immunosuppressive drugs used to prevent or treat organ or bone-marrow allograft rejection, graft versus host disease, and autoimmune diseases. However, animal-derived glycoproteins are also strongly immunogenic and rabbit ATG induces
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