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I8523

Sigma-Aldrich

Interleukin-12 from mouse

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

IL-12

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10 μG
$405.30

$405.30

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Estimated to ship onJune 16, 2025Details

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10 μG
$405.30

About This Item

MDL number:
MFCD00212975
UNSPSC Code:
12352202
NACRES:
NA.32

$405.30

List Price$579.00Save 30%
Web-Only Promotion

Estimated to ship onJune 16, 2025Details

biological source

mouse

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.01-.1 ng/mL ED50

mol wt

glycoprotein 70 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P43431, P43432

storage temp.

−20°C

Gene Information

mouse ... Il12a(16159), Il12b(16160)

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This Item
I2276I4026H7666
Interleukin-12 from mouse ≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

I8523

Interleukin-12 from mouse

Interleukin-12 human ≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

I2276

Interleukin-12 human

Interleukin-17 from mouse ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

I4026

Interleukin-17 from mouse

Interleukin-12 human HumanKine®, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin, tested

H7666

Interleukin-12 human

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

biological source

mouse

biological source

human

biological source

mouse

biological source

human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized

Application

Interleukin-12 (IL-12) from mouse has been used to study the effect of IL-12 on Th1 (type 1 T helper) immunity in Mongolian gerbils (Meriones unguiculatus) infected with Sporothrix schenckii.[1] It has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.[2]

Biochem/physiol Actions

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCT8244
MKCT2909
MKCV1726
MKCQ8261
MKCQ5395

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Morgane Boulch et al.
Nature cancer, 4(7), 968-983 (2023-05-30)
CD4+ T cells and CD4+ chimeric antigen receptor (CAR) T cells display highly variable antitumor activity in preclinical models and in patients; however, the mechanisms dictating how and when CD4+ T cells promote tumor regression are incompletely understood. With the
Morgane Boulch et al.
Science immunology, 6(57) (2021-03-28)
Chimeric antigen receptor (CAR) T cell therapy relies on the activity of a large pool of tumor-targeting cytotoxic effectors. Whether CAR T cells act autonomously or require interactions with the tumor microenvironment (TME) remains incompletely understood. Here, we report an
Aurelio Flores-García et al.
Journal of chemotherapy (Florence, Italy), 27(2), 87-93 (2014-02-20)
Sporotrichosis is a cutaneous fungal infection caused by Sporothrix schenckii. It is known to be mainly contained by Th1 responses. As IL-12 is crucial for Th1 response, we investigated if treatment with recombinant murine IL-12 (rmIL-12) promoted Th1 immunity and/or
Heather M McGee et al.
Immunology and cell biology, 89(7), 767-776 (2011-01-19)
Neonates have a developing immune response, with a predisposition towards induction of tolerance. As the immune system develops, immunity rather than tolerance is induced, with this development of immunity occurring in response to external factors such as the environment. As

Articles

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