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I8648

Sigma-Aldrich

Interleukin-15 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
IL-15
MDL number:

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.2-4.0 ng/mL EC50

mol wt

antigen predicted mol wt ~12.5 kDa

packaging

pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL15(3600)

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Interleukin-10 human

recombinant

expressed in E. coli

recombinant

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recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

assay

>97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

antigen predicted mol wt ~12.5 kDa

mol wt

32-34 kDa

mol wt

18 kDa

mol wt

45-50 kDa

packaging

pkg of 5 μg

packaging

pkg of 5 μg

packaging

pkg of 5 μg

packaging

pkg of 5 μg

Biochem/physiol Actions

IL-15 (interleukin 15) is a proinflammatory pleiotropic cytokine and is a connection between innate and adaptive immune responses. It participates in the proliferation, survival and activation of various lymphocytes lineages. IL-15 works by associating with a high affinity heterotrimeric receptor complex containing IL-15Rα, IL-2/15Rβ and IL-Rγ. It is present in different forms, the soluble form, the hyper IL-15 formed by association between IL-15 receptor α chain and soluble cytokine, and the membrane bound isoform.
Interleukin-15 was first isolated from the supernatant of a cultured simian kidney epithelial cell line. The cDNA encodes a 162 amino acid precursor protein with a 48 amino acid signal peptide that is cleaved to yield a 114 amino acid mature protein. Human IL-15 shares sequence identity with simian (~97%) and murine (~73%). IL-15 competes for binding sites with IL-2, as both IL-2 and IL-15 stimulate the growth of cells through the IL-2 receptor.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline, pH 7.4 with 50 μg bovine serum albumin per 1 μg as a carrier protein.

Analysis Note

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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