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I9032

Sigma-Aldrich

Interferon β from mouse

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

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Synonym(s):
IFN β
MDL number:
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in E. coli

assay

≥95%

form

buffered aqueous solution

mol wt

~20 kDa

packaging

pkg of 1 vial

concentration

2560000 units/mL

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... Ifnb1(15977)

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vibrant-m

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Interleukin-1β from mouse

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

mouse

biological source

mouse

biological source

rat

biological source

mouse

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in CHO cells

recombinant

expressed in E. coli

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

form

lyophilized powder

General description

IFN β (interferon β) is from the type 1 interferon family of cytokines.
Interferon β (IFN-β) possesses a signal peptide region. It has comparatively more hydrophobic residues and displays 30% homology with IFN-α.
The gene encoding interferon β (Infb1) is localized on mouse chromosome 4.

Application

Interferon β from mouse has been used:
  • in the stimulation of bone marrow-derived dendritic cells (BMDCs)
  • as a cytokine to stimulate differentiation of podocytes
  • to stimulate splenocytes

Interferon ß from mouse has been used:
  • to induce the activation of cholesterol 25-hydroxylase, by in vitro stimulation of bone marrow-derived macrophages, dendritic cells and CD11c+ lung macrophages
  • to stimulate splenocytes, in order to study the secretion and regulation of IFN-γ
  • to intranasally pre-treat neonatal mice, to study its effects on the protective actions of Lactobacillus rhamnosus GG (LGG)
  • as a standard to treat MEF-Mx2-luc-BKO reporter cell line for IFN-β measurement, in response to human adenovirus-5 (HAdV-C5)

Biochem/physiol Actions

In presence of virus infection, IFN β (interferon β) is upregulated and plays a crucial role in innate antiviral response. It is also important for anti-inflammatory effects.
Interferon β (IFN-β) suppresses a variety of proinflammatory cytokines in lymphocytes including the interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α). It also favors the synthesis of antagonists associated with interleukin IL-10 and IL-1 receptor. IFN-β downregulates interferon γ production but enhances transforming growth factor β1 expression. It has therapeutic potential in treating rheumatoid arthritis and relapsing-remitting multiple sclerosis (MS).
Interferon β has a role in the activation of human leukocyte antigen (HLA) gene expression. It acts as a messenger during bacterial infections.
Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Physical form

Solution in 20 mM HEPES, pH 6.0, 0.5 M NaCl, 6% glycerol, and 0.1% bovine serum albumin.

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using mouse L929 cells with encephalomyocarditis virus. The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for mouse interferon β.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Manuel Hagen et al.
Cell cycle (Georgetown, Tex.), 15(14), 1929-1937 (2016-05-28)
Podocytes are terminally differentiated renal cells, lacking the ability to regenerate by proliferation. However, during renal injury, podocytes re-enter into the cell cycle but fail to divide. Earlier studies suggested that re-entry into cell cycle results in loss of podocytes
P Keskinen et al.
Immunology, 91(3), 421-429 (1997-07-01)
HLA class I and II molecules play a central role in regulating host immune responses against microbial infections because they present foreign antigens to CD8+ and CD4+ T lymphocytes, respectively. Many cytokines, especially interferons (IFN), are known to upregulate human
Nanthapon Ruangkiattikul et al.
Virulence, 8(7), 1303-1315 (2017-04-20)
Type I interferons (IFN-I), such as IFN-α and IFN-β are important messengers in the host response against bacterial infections. Knowledge about the role of IFN-I in infections by nontuberculous mycobacteria (NTM) is limited. Here we show that macrophages infected with
ENU-induced mutation in USP18 causes hyperactivation of IFN-alphabeta signalling and suppresses STAT4-induced IFN-gamma production resulting in increased susceptibility to Salmonella Typhimurium
Richer E, et al.
Journal of immunology (Baltimore, Md. : 1950), 185(6), 3593-3593 (2010)
O I Olopade et al.
Cancer research, 53(10 Suppl), 2410-2415 (1993-05-15)
Cytogenetic analyses of non-small cell lung cancer have revealed deletions of the short arm of chromosome 9 with breakpoints at 9p11-pter in a significant proportion of tumors. Recent evidence suggests that homozygous loss of the interferon (IFN) and methylthioadenosine phosphorylase

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