I9032

Interferon β from mouse

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

• Synonym(s): IFN β
• MDL number: MFCD02189136
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in E. coli
• assay: ≥95%
• form: buffered aqueous solution
• mol wt: ~20 kDa
• packaging: pkg of 1 vial
• concentration: 2560000 units/mL
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P01575
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: mouse ... Ifnb1(15977)

Description

General description

IFN β (interferon β) is from the type 1 interferon family of cytokines.[1]

Interferon β (IFN-β) possesses a signal peptide region. It has comparatively more hydrophobic residues and displays 30% homology with IFN-α.[2]

The gene encoding interferon β (Infb1) is localized on mouse chromosome 4.[3]

Application

Interferon β from mouse has been used:

• in the stimulation of bone marrow-derived dendritic cells (BMDCs)[4]
• as a cytokine to stimulate differentiation of podocytes[5]
• to stimulate splenocytes[6]

Interferon ß from mouse has been used:

• to induce the activation of cholesterol 25-hydroxylase, by in vitro stimulation of bone marrow-derived macrophages, dendritic cells and CD11c⁺ lung macrophages[4]
• to stimulate splenocytes, in order to study the secretion and regulation of IFN-γ [7]
• to intranasally pre-treat neonatal mice, to study its effects on the protective actions of Lactobacillus rhamnosus GG (LGG)[8]
• as a standard to treat MEF-Mx2-luc-BKO reporter cell line for IFN-β measurement, in response to human adenovirus-5 (HAdV-C5)[9]

Biochem/physiol Actions

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

In presence of virus infection, IFN β (interferon β) is upregulated and plays a crucial role in innate antiviral response.[10] It is also important for anti-inflammatory effects.[1]

Interferon β (IFN-β) suppresses a variety of proinflammatory cytokines in lymphocytes including the interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α).[11] It also favors the synthesis of antagonists associated with interleukin IL-10 and IL-1 receptor.[11] IFN-β downregulates interferon γ production but enhances transforming growth factor β1 expression.[11] It has therapeutic potential in treating rheumatoid arthritis[11] and relapsing-remitting multiple sclerosis (MS).[12]

Interferon β has a role in the activation of human leukocyte antigen (HLA) gene expression.[13] It acts as a messenger during bacterial infections.[14]

Physical form

Solution in 20 mM HEPES, pH 6.0, 0.5 M NaCl, 6% glycerol, and 0.1% bovine serum albumin.

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using mouse L929 cells with encephalomyocarditis virus. The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for mouse interferon β.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)