I9135

Sigma-Aldrich

Anti-Human IgG (Fc specific) antibody produced in rabbit

whole antiserum

Synonym(s):
Rabbit Anti Human IgG - Anti-Human IgG (Fc specific) antibody produced in rabbit, Rabbit Anti Human Igg, Rabbit Antiserum, Human Antiserum
MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

whole antiserum

antibody product type

secondary antibodies

clone

polyclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:100,000
quantitative precipitin assay: 1.5 mg/mL

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

IgG contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that are associated by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Anti-Human IgG (Fc specific) antibody produced in rabbit was used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a pivotal role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be linked to allergy and has the longest serum half life.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Preparation Note

treated to remove lipoproteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

John D Bartlett et al.
Cells, tissues, organs, 194(2-4), 222-226 (2011-04-29)
Dental enamel development occurs in stages as observed by the changing morphology of the ameloblasts that are responsible for enamel formation. During the secretory stage of development, proteins including MMP20 are secreted into the enamel matrix. MMP20 is required for...
Kimberly M Stroka et al.
Blood, 118(6), 1632-1640 (2011-06-10)
A vast amount of work has been dedicated to the effects of shear flow and cytokines on leukocyte transmigration. However, no studies have explored the effects of substrate stiffness on transmigration. Here, we investigated important aspects of endothelial cell contraction-mediated...
Puja Gupta et al.
BMC biotechnology, 10, 72-72 (2010-10-12)
EGF receptor variant III (EGFRvIII) is the most common variant of the EGF receptor observed in human tumors. It results from the in frame deletion of exons 2-7 and the generation of a novel glycine residue at the junction of...
Franco Klingberg et al.
The Journal of cell biology, 207(2), 283-297 (2014-10-22)
Integrin-mediated force application induces a conformational change in latent TGF-β1 that leads to the release of the active form of the growth factor from the extracellular matrix (ECM). Mechanical activation of TGF-β1 is currently understood as an acute process that...
Keiko Matsuda et al.
The European journal of neuroscience, 33(8), 1447-1461 (2011-03-18)
Cbln1 (a.k.a. precerebellin) is a unique bidirectional synaptic organizer that plays an essential role in the formation and maintenance of excitatory synapses between granule cells and Purkinje cells in the mouse cerebellum. Cbln1 secreted from cerebellar granule cells directly induces...

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