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I9154

Sigma-Aldrich

Interleukin-10 from rat

>95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

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Synonym(s):
IL-10
EC Number:
MDL number:
NACRES:
NA.32

biological source

rat

Quality Level

recombinant

expressed in E. coli

Assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

1-35 ng/mL ED50

mol wt

predicted mol wt ~19 kDa

packaging

pkg of 5 μg

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

rat ... Il10(25325)

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I3019I2276I3901
Sigma-Aldrich

Sigma-Aldrich

I2276

Interleukin-12 human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in E. coli

assay

>95% (SDS-PAGE)

assay

>97% (SDS-PAGE)

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

predicted mol wt ~19 kDa

mol wt

~18 kDa

mol wt

glycoprotein 75 kDa

mol wt

~18 kDa

packaging

pkg of 5 μg

packaging

pkg of 5 μg

packaging

pkg of 5 μg

packaging

pkg of 5 μg

Application

Interleukin-10 (IL-10) from rat has been used to study the anti-allodynic effect of IL-10 in rat model.

Biochem/physiol Actions

Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Physical form

Lyophilized from a 0.2 μm filtered in 20 mM Tris, 0.1 M sodium chloride containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using the murine mast cell line, MC/9.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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We examined the possible anti-inflammatory mechanisms of gabapentin in the attenuation of neuropathic pain and the interaction between the anti-allodynic effects of gabapentin and interleukin-10 (IL-10) expression in a rat model of neuropathic pain. The anti-allodynic effect of intrathecal gabapentin
Justine Durand et al.
Journal of immunology (Baltimore, Md. : 1950), 195(10), 5035-5044 (2015-10-04)
Emerging knowledge regarding B cells in organ transplantation has demonstrated that these cells can no longer be taken as mere generators of deleterious Abs but can also act as beneficial players. We previously demonstrated in a rat model of cardiac
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Fucosylated glycans on the surface of epithelial cells (ECs) regulate intestinal homeostasis by serving as attachment receptors and a nutrient source for some species of bacteria. We show here that epithelial fucosylation in the ileum is negatively regulated by IL-10-producing
Martina Severa et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 35(9), 668-681 (2015-04-30)
Plasmacytoid dendritic cells (pDCs) display altered immune-phenotype in multiple sclerosis (MS) patients and are found actively recruited in postmortem MS brain lesions, implying that their immune regulation may represent an important aspect of MS pathogenesis. Because of the reported Toll-like
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Immunity to malaria can be acquired but only after repeat exposures to polymorphic Plasmodium. However, the development of clinical outcomes during P. falciparum infection is not clearly understood. This study elucidated whether monocytes, neutrophils and pro/anti-inflammatory cytokines were associated with

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