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I9388

Sigma-Aldrich

Monoclonal Anti-Human IgG1 (Fc specific) antibody produced in mouse

clone HP-6001, ascites fluid

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.77

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6001, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:3,000

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders
Monoclonal Anti-Human IgG1 (Fc specific) antibody is specific for an epitope in the Fc region of human IgG1 and does not detect IgG2, IgG3, and IgG4. The association constant of this antibody with its ligand is estimated to be 1x(107) L/M. This product clone has been tested for specificity using various immunoassays in the IUIS/WHO study.

Specificity

Monoclonal Anti-Human IgG1 (Fc specific) is specific for the IgG1 subclass and nonreactive with IgG2, IgG3, and IgG4 in an ELISA. The antibody recognizes an epitope expressed in the Fc region of IgG1. The estimated association constant of this antibody with its ligand is 1x107 L/M.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

Monoclonal Anti-Human IgG1 (Fc specific) antibody is suitable for use in ELISA and immunoblot.

Biochem/physiol Actions

IgG1 has a molecular weight of 146 kDa and is an abundantly expressed IgG subclass. IgG1 deficiencies contribute to overall decrease in total IgG levels resulting in hypogammaglobulinemia. IgG1 promotes bacterial phagocytosis and mediates vaccine-induced protection from infection. The ratio of IgG2a to IgG1 is crucial for immune response against Leishmania tropica infection. It has high binding affinity towards the Fcγ receptors (FcγR).

Physical form

Monoclonal Anti-Human IgG1 (Fc specific) is provided as ascites fluid with 15 mM sodium azide.

Storage and Stability

Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chia-Wen Liu et al.
Frontiers in immunology, 12, 773013-773013 (2021-12-21)
Gardnerella vaginalis is associated with bacterial vaginosis (BV). The virulence factors produced by G. vaginalis are known to stimulate vaginal mucosal immune response, which is largely driven by activated macrophages. While Tilapia piscidin 4 (TP4), an antimicrobial peptide isolated from
Negative Immunodiffusion Test Results Obtained with Sera of Paracoccidioidomycosis Patients May Be Related to Low-Avidity Immunoglobulin G2 Antibodies Directed against Carbohydrate Epitopes
Neves, A., R., et al.
Clinical and Vaccine Immunology : CVI, 10(5), 802-807 (2003)
Lower levels of IgG1 in comparison with IgG2a are associated with protective immunity against Leishmania tropica infection in BALB/c mice.
Rostamian M, et al.
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi, 50(2), 160-166 (2017)
IgG1 is required for optimal protection after immunization with the purified porin OmpD from Salmonella Typhimurium.
Zhang Y, et al.
Journal of Immunology, 199(12), 4103-4109 (2017)
Z H Wang et al.
The Journal of infectious diseases, 181(2), 456-462 (2000-02-11)
Isotype-specific serum antibody responses against human papillomavirus (HPV) type 16 were evaluated by use of cross-sectional, prospective, and population-based seroepidemiologic studies. IgG1 and IgA were the most abundant isotypes. No sample contained IgG2, and <25 samples contained IgG3 or IgM.

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