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I9392

Sigma-Aldrich

3-Methylbutanol

BioReagent, for molecular biology, ≥98.5%

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Synonym(s):
3-Methyl-1-butanol, Isoamyl alcohol, Isopentyl alcohol
Linear Formula:
(CH3)2CHCH2CH2OH
CAS Number:
Molecular Weight:
88.15
Beilstein:
1718835
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

product line

BioReagent

Assay

≥98.5%

form

liquid

technique(s)

RNA extraction: suitable

pH

5.6 (20 °C, 25 g/L)

density

0.809 g/mL at 20 °C (lit.)

suitability

suitable for nucleic acid purification

storage temp.

room temp

SMILES string

CC(C)CCO

InChI

1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3

InChI key

PHTQWCKDNZKARW-UHFFFAOYSA-N

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This Item
320021AX144059085
3-Methylbutanol BioReagent, for molecular biology, ≥98.5%

Sigma-Aldrich

I9392

3-Methylbutanol

-
3-Methyl-1-butanol ACS reagent, ≥98.5%

Sigma-Aldrich

320021

3-Methyl-1-butanol

Essential Grade
Isoamyl Alcohol Meets ACS Specifications GR ACS

Supelco

AX1440

Isoamyl Alcohol

-
3-Methylbutanol BioUltra, for molecular biology, ≥99.0% (GC)

Sigma-Aldrich

59085

3-Methylbutanol

Premium Grade
assay

≥98.5%

assay

≥98.5%

assay

≥98.5% (corrected for water content, GC)

assay

≥99.0% (GC)

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

RNA extraction: suitable

technique(s)

-

technique(s)

mass spectrometry (MS): suitable

technique(s)

DNA extraction: suitable, RNA extraction: suitable

pH

5.6 (20 °C, 25 g/L)

pH

5.6 (20 °C, 25 g/L)

pH

7 (20 °C, 25 g/L in H2O)

pH

5.6 (20 °C, 25 g/L)

density

0.809 g/mL at 20 °C (lit.)

density

0.809 g/mL at 25 °C (lit.)

density

0.81 g/cm3 at 20 °C, 0.809 g/mL at 25 °C (lit.)

density

0.809 g/mL at 20 °C (lit.)

General description

3-methylbutanol (isoamyl alcohol) is a clear, colorless alcohol commonly used as an organic solvent . It can also be used in phenol-chloroform nucleic acid extraction and purification protocols, where it inhibits RNase activity and reduces foaming. Isoamyl alcohol can also be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation.

Application

3-Methylbutanol (isoamyl alcohol) in combination with phenol and chloroform is used in extraction of RNA. It was used in the synthesis of oligo-RNAs using photocaged adenosine molecules.
3-Methylbutanol has been used as a component of phenol/chloroform/ isoamyl alcohol solution for DNA extraction from blood and from absorbing substrata based forensic samples and biofilms.

related product

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

110.3 °F - closed cup

Flash Point(C)

43.5 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Section 1 Isolation of nucleic acids
Biofouling Methods, 88-88 (2014)
DNA extraction from blood and forensic samples
Guidelines for Molecular Analysis in Archive Tissues, 45-54 (2011)
Steven G Chaulk et al.
Nature protocols, 2(5), 1052-1058 (2007-06-05)
This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the
Kazushi Yoshida et al.
Nature communications, 3, 739-739 (2012-03-15)
The same odorant can induce attractive or repulsive responses depending on its concentration in various animals including humans. However, little is understood about the neuronal basis of this behavioural phenomenon. Here we show that Caenorhabditis elegans avoids high concentrations of
Frédéric Ravyts et al.
Food microbiology, 27(7), 945-954 (2010-08-07)
Differences in the production of bacterial metabolites with potential impact on fermented sausage flavour were found in meat simulation medium when comparing different strains of Staphylococcus xylosus and Staphylococcus carnosus as starter cultures. Overall, higher levels of 3-methyl-1-butanol and acetoin

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