I9392

Sigma-Aldrich

3-Methylbutanol

BioReagent, for molecular biology, ≥98.5%

Synonym(s):
Isopentyl alcohol, Isoamyl alcohol, 3-Methyl-1-butanol
Linear Formula:
(CH3)2CHCH2CH2OH
CAS Number:
Molecular Weight:
88.15
Beilstein/REAXYS Number:
1718835
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

Quality Level

grade

for molecular biology

product line

BioReagent

assay

≥98.5%

form

liquid

application(s)

RNA extraction: suitable

pH

5.6 (20 °C, 25 g/L)

density

0.809 g/mL at 20 °C (lit.)

suitability

suitable for nucleic acid purification

storage temp.

room temp

SMILES string

CC(C)CCO

InChI

1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3

InChI key

PHTQWCKDNZKARW-UHFFFAOYSA-N

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General description

3-methylbutanol (isoamyl alcohol) is a clear, colorless alcohol commonly used as an organic solvent . It can also be used in phenol-chloroform nucleic acid extraction and purification protocols, where it inhibits RNase activity and reduces foaming. Isoamyl alcohol can also be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation.

Application

3-Methylbutanol has been used as a component of phenol/chloroform/ isoamyl alcohol solution for DNA extraction from blood and from absorbing substrata based forensic samples and biofilms.
3-Methylbutanol (isoamyl alcohol) in combination with phenol and chloroform is used in extraction of RNA. It was used in the synthesis of oligo-RNAs using photocaged adenosine molecules.

Packaging

1 L in glass bottle
25, 500 mL in glass bottle

Signal Word

Danger

Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

UN1105 - class 3 - PG 3 - Pentanols

WGK Germany

WGK 1

Flash Point(F)

109.4 °F - closed cup

Flash Point(C)

43 °C - closed cup

Certificate of Analysis

Certificate of Origin

DNA extraction from blood and forensic samples
Guidelines for Molecular Analysis in Archive Tissues, 45-54 (2011)
Steven G Chaulk et al.
Nature protocols, 2(5), 1052-1058 (2007-06-05)
This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the...
Section 1 Isolation of nucleic acids
Biofouling Methods, 88-88 (2014)
Mary J Dunlop et al.
Molecular systems biology, 7, 487-487 (2011-05-11)
Many compounds being considered as candidates for advanced biofuels are toxic to microorganisms. This introduces an undesirable trade-off when engineering metabolic pathways for biofuel production because the engineered microbes must balance production against survival. Cellular export systems, such as efflux...
Niels O Verhulst et al.
Malaria journal, 10, 28-28 (2011-02-10)
Anopheles gambiae sensu stricto is considered to be highly anthropophilic and volatiles of human origin provide essential cues during its host-seeking behaviour. A synthetic blend of three human-derived volatiles, ammonia, lactic acid and tetradecanoic acid, attracts A. gambiae. In addition...

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