I9885

Sigma-Aldrich

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment antibody produced in goat

affinity isolated antibody, lyophilized powder

MDL number:
NACRES:
NA.46

Quality Level

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

packaging

pkg of 5x1 mg
pkg of 1 mg

storage condition

avoid repeated freeze/thaw cycles

application(s)

Ouchterlony double diffusion: suitable
quantitative precipitin assay: suitable

color

white to off-white

solubility

water: soluble, clear to hazy, colorless (after reconstitution with 1 ml of 0.135 M NaCl)

conjugate

unconjugated

storage temp.

2-8°C

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Human IgG (γ-chain specific), F(ab′)2 fragment antibody is specific for human IgG subclasses. The F(ab′)2 fragment of the antibody is obtained from pepsin-digested antiserum by immunospecific methods of purification.

Immunogen

Purified human IgG

Application

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment antibody is suitable for Ouchterlony double diffusion and quantitative precipitin assay. The antibody was used in ELISA and to produce Fab′-oligonucleotide conjugates.

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
null
Z N Chaudri et al.
FEBS letters, 450(1-2), 23-26 (1999-06-01)
The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of...
H Brandstätter et al.
Vox sanguinis, 103(3), 201-212 (2012-04-14)
Complement factor H (CFH) acts as major regulator of the alternative pathway of complement and its mutations and polymorphisms predispose to various human diseases. We aimed to develop a scalable purification process of CFH from human plasma fractions to supply...
Francesco Lolli et al.
Journal of neuroimmunology, 167(1-2), 131-137 (2005-07-30)
Synthetic glycopeptides have the potential to detect antibodies in multiple sclerosis (MS). In the present study, we analyzed the antibodies (IgM class, IgG class and IgG subclasses) to the synthetic glycopeptide CSF114(Glc) in the serum of 186 MS patients, 166...
Christian Loch et al.
Journal of pharmaceutical sciences, 103(2), 517-526 (2013-12-07)
Intravitreal injections and drug-loaded implants are current approaches to treat diseases of the posterior eye. To investigate the release of active agents and their distribution in the vitreous body, a new test system was developed that enables a realistic simulation...

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