I9888

Sigma-Aldrich

Monoclonal Anti-Human IgG4 (pFc′ specific) antibody produced in mouse

clone HP-6023, ascites fluid

MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6023, monoclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:2,000

isotype

IgG3

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Monoclonal Anti-Human IgG4 (pFc′ specific) antibody is specific for an epitope on the pFc′ region of IgG4. The product does not react with IgG1, IgG2 and IgG3. This monoclonal antibody has been cited as the most widely applicable IgG4 specific reagent by the IUIS/WHO study.
An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. Human IgG4 is rarely found in the serum. It has the ability to bind FcγRI with high affinity. High levels of IgG4 in the serum can be pathogenic and may lead to Wegener′s granulomatosis.
IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

Monoclonal Anti-Human IgG4 (pFc′ specific) antibody can be used for immunoblotting and Indirect Immunoperoxidase Assay.
This clone has been singled out as most widely applicable by the IUIS/WHO study.
Monoclonal Anti-Human IgG4 (pFc′ specific) antibody has been used in immunoblotting.

Biochem/physiol Actions

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differs from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

D Bencina et al.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 19(7), 521-530 (2000-09-01)
Mycoplasma pneumoniae commonly causes respiratory tract infections in humans, but it may also be associated with central nervous system manifestations. The aim of the present study was to determine whether the cerebrospinal fluid taken from patients with neurologic symptoms due...
Negative immunodiffusion test results obtained with sera of paracoccidioidomycosis patients may be related to low-avidity immunoglobulin G2 antibodies directed against carbohydrate epitopes
Neves AR, et al.
Clinical and Diagnostic Laboratory Immunology, 10(5), 802-807 (2003)
Negative Immunodiffusion Test Results Obtained with Sera of Paracoccidioidomycosis Patients May Be Related to Low-Avidity Immunoglobulin G2 Antibodies Directed against Carbohydrate Epitopes
Neves, A., R., et al.
Clinical and Vaccine Immunology : CVI, 10(5), 802-807 (2003)
Roald N
The Immunoglobulins: Structure and Function (1998)
Anti-inflammatory activity of human IgG4 antibodies by dynamic Fab arm exchange.
Van Der Neut Kolfschoten M, et al.
Science, 317(5844), 1554-1557 (2007)
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
Read More

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