MilliporeSigma
All Photos(1)

ISO2

Sigma-Aldrich

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Sign Into View Organizational & Contract Pricing

Select a Size

MDL number:
NACRES:
NA.32

usage

sufficient for 1000 tests (clones) (by ELISA)
sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Quality Level

shipped in

wet ice

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C7988A7554SAB4200669
shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Specificity

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Application

Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays.
Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.

Features and Benefits

  • May be used in a variety of assay formats
  • Suitable for all antibody forms
  • Determines all mouse IgG subclasses, IgA, and IgM

Kit Components Only

Product No.
Description

  • goat antisera to mouse IgA .2 mL

  • goat antisera to mouse IgG3 .2 mL

  • goat antisera to mouse IgG1 .2 mL

  • goat antisera to mouse IgG2a .2 mL

  • goat antisera to mouse IgG2b .2 mL

  • goat antisera to mouse IgM .2 mL

related product

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Yuan Dong et al.
SLAS discovery : advancing life sciences R & D, 25(3), 310-319 (2019-09-29)
D-dimer is an essential diagnostic index of thrombotic diseases. Since the existing anti-D-dimer antibodies vary in quality and specificity, a search for alternative anti-D-dimer antibodies is required. The present study aimed to screen a novel monoclonal antibody (mAb) against D-dimer
Generation and characterization of novel stromal specific antibodies
Halder S, et al.
Cell Research, 15(9), 739-739 (2005)
Qimin Wang et al.
Frontiers in microbiology, 11, 1961-1961 (2020-09-15)
The extracellular domain of influenza M2 protein (M2e) is highly conserved and is a promising target for development of universal influenza vaccines. Here, we synthesized a peptide vaccine consisting of M2e epitope linked to a fibrillizing peptide, which could self-assemble
Olga V Fedorova et al.
Journal of hypertension, 26(12), 2414-2425 (2008-11-15)
Levels of marinobufagenin (MBG), an endogenous bufadienolide Na/K-ATPase (NKA) inhibitor, increase in preeclampsia and in NaCl-sensitive hypertension. We tested a 3E9 monoclonal anti-MBG antibody (mAb) for the ability to lower blood pressure (BP) in NaCl-sensitive hypertension and to reverse the
Preparation and Characterization of a Monoclonal Antibody with High Affinity for Soluble A β Oligomers
Ying,Z et al.
Hybridoma, 28(5) (2009)

Protocols

ELISA Procedures

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service