This product may be stored at room temperature in powdered form. Solutions are stable at 37 °C for approximately 5 days. Aqueous stock solutions may be stored at 2-8 °C for long term storage. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/666/560/k1377pis.pdf
K1377
Kanamycin sulfate from Streptomyces kanamyceticus
powder, BioReagent, suitable for cell culture, suitable for plant cell culture
Synonym(s):
Kanamycin A, Kanamycin sulfate salt
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About This Item
Empirical Formula (Hill Notation):
C18H36N4O11 · H2O4S
CAS Number:
Molecular Weight:
582.58
EC Number:
MDL number:
UNSPSC Code:
51281654
PubChem Substance ID:
NACRES:
NA.76
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biological source
Streptomyces kanamyceticus
product line
BioReagent
form
powder
potency
≥750 μg per mg (dry basis)
technique(s)
cell culture | mammalian: suitable
cell culture | plant: suitable
color
white to off-white
solubility
H2O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma
application(s)
agriculture
mode of action
protein synthesis | interferes
SMILES string
OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
InChI
1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
InChI key
OOYGSFOGFJDDHP-KMCOLRRFSA-N
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This Item | K0254 | 420311 | K0129 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable, cell culture | plant: suitable |
| mode of action protein synthesis | interferes | mode of action protein synthesis | interferes | mode of action - | mode of action protein synthesis | interferes |
| antibiotic activity spectrum Gram-negative bacteria, mycobacteria, Gram-positive bacteria, mycoplasma | antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma | antibiotic activity spectrum - | antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| product line BioReagent | product line BioReagent | product line - | product line BioReagent |
| application(s) agriculture | application(s) - | application(s) - | application(s) - |
General description
Chemical structure: aminoglycoside
Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100μg/mL.
Application
Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.[1]
Biochem/physiol Actions
Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.
Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.
Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.
Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.
Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.
Preparation Note
Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.
Disclaimer
Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 2
ppe
Eyeshields, Gloves, type N95 (US)
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Product Information Sheet
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What temperature should this product be stored at?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the difference between K1377 and K4000? K4000 is labeled as being free of animal-derived materials, but K1377 contains them?
1 answer-
Both the K1377 and K4000 are animal component free. They are the same compound with the same properties, however the K1377 is also tested for use in mammalian and plant cell culture. See the links below for a sample Certificate of Analysis for each for comparison:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/K1377-5GSLCM4121.pdf
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/K4000-BULKSLCM4121.pdfHelpful?
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