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K2019

Sigma-Aldrich

Anti-PIP5K1C (N-terminal) antibody produced in rabbit

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-LCCS3, Anti-Phosphatidylinositol-4-phosphate 5-kinase, type I, γ, Anti-PIP5Kγ
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~90 kDa

species reactivity

human, mouse, rat

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

application(s)

immunoprecipitation (IP): 5-10 μg using mouse brain extract (S1 fraction).
western blot: 0.5-1 μg/mL using HEK-293T cells expressing human PIP5K1C
western blot: 1-2 μg/mL using rat brain (S1 fraction)

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... PIP5K1C(23396)
mouse ... Pip5k1c(18717)
rat ... Pip5k1c(314641)

General description

Phosphatidylinositol-4-phosphate-5-kinase, type 1γ (PIP5K1C) belongs to the type I phosphatidylinositol-4-phosphate-5-kinases. This protein is expressed at high levels in the brain and is concentrated at synapses. The PIP5K1C gene is located on the human chromosome at 19p13.3.

Specificity

Anti-PIP5K1C (N-terminal) specifically recognizes human, rat, and mouse PIP5K1C.

Application

Anti-PIP5K1C (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Phosphatidylinositol-4-phosphate-5-kinase, type 1γ (PIP5K1C) is involved in phosphorylating phosphatidylinositol-4-phosphate (PtdInsP) to generate phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2). PIP5K1C has been shown to bind to the 4.1/ezrin/radixin/moesin (FERM) domain of talin, which contains also the binding sites of β-integrin, actin, and PtdIns(4,5)P2. PIP5K1C is recruited to the membrane by talin at neuronal synapses may be required to generate the PtdIns(4,5)P2 pool involved in clathrin coating and actin dynamics during vesicle recycling. Mutations in the PIP5K1C gene is associated with lethal congenital contracture syndrome type 3 (LCCS3), which is an intense form of arthrogryposis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

1

Certificate of Analysis
Certificate of Origin
Guiqing Lu et al.
American journal of cancer research, 10(10), 3382-3394 (2020-11-10)
Endosomes regulate cell polarity, adhesion, signaling, immunity, and tumor progression, which may influence cancer outcomes. Here we evaluated associations between 36,068 genetic variants of 228 endosome-related pathway genes and cutaneous melanoma disease-specific survival (CMSS) using genotyping data from two previously...
Ginat Narkis et al.
American journal of human genetics, 81(3), 530-539 (2007-08-19)
Lethal congenital contractural syndrome (LCCS) is a severe form of arthrogryposis. To date, two autosomal recessive forms of the disease (LCCS and LCCS2) have been described and mapped to chromosomes 9q34 and 12q13, respectively. We now describe a third LCCS...
Gilbert Di Paolo et al.
Nature, 420(6911), 85-89 (2002-11-08)
Membrane phosphoinositides control a variety of cellular processes through the recruitment and/or regulation of cytosolic proteins. One mechanism ensuring spatial specificity in phosphoinositide signalling is the targeting of enzymes that mediate their metabolism to specific subcellular sites. Phosphatidylinositol phosphate kinase...
Alexander Wallroth et al.
The Journal of biological chemistry, 293(5), 1526-1535 (2017-12-29)
Phosphoinositides (PIs) are phospholipids that perform crucial cell functions, ranging from cell migration and signaling to membrane trafficking, by serving as signposts of compartmental membrane identity. Although phosphatidylinositol 4,5-bisphosphate, 3-phosphate, and 3,5-bisphosphate are commonly considered as hallmarks of the plasma...

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