Unfortunately, a Krebs-Ringer Bicarbonate Buffer without glucose is not available.
K4002
Krebs-Ringer Bicarbonate Buffer
With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
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Product Name
Krebs-Ringer Bicarbonate Buffer, With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
pH
6.1-6.7
solubility
water: 9.5 g/L, clear
storage temp.
2-8°C
Quality Level
1 of 4
This Item | K3753 | T2397 | H1387 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level 500 |
| form powder | form powder | form liquid | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C |
| solubility water: 9.5 g/L, clear | solubility - | solubility - | solubility - |
| pH 6.1-6.7 | pH - | pH - | pH - |
Application
General description
Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 1.26 g/L sodium bicarbonate.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Related Content
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Do you have a glucose free product?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should Product K4002, Krebs-Ringer Bicarbonate Buffer, be dissolved?
1 answer-
Product No. K4002 is listed in a 10X1L package size, meaning that each of the ten containers has enough powder to make 1 Liter of KRB buffer. It can be dissolved in 1 Liter of either water or medium.
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What is the formula of Product K4002, Krebs-Ringer Bicarbonate Buffer (KRB)?
1 answer-
The formulation for KRB can be found on the data sheet.
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Product K4002 - Krebs-Ringer Bicarbonate Buffer, is without calcium chloride and sodium bicarbonate. In the reconstitution steps, you advice to add 1.26g sodium bicarbonate. Should we also add some calcium chloride? in which amount?
1 answer-
Product K4002 - Krebs-Ringer Bicarbonate Buffer does not contain calcium. The addition of calcium is not required for osmolarity. If you need to have calcium, you can add calcium chloride between 1.25 to 2.5 mM. You may want to check the osmolarity and make sure it is not too high.Kreb Henseleit Buffer has 1.25 mM calcium chlorideKreb's Buffer has 2.5 mM calcium chlorideKreb' Ringer Superfusion solution contains 2.0 mM calcium chloride.Please see the attached document for different Krebs' buffer recipes.
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