K4143

PKCη, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): MG C26269, MG C5363, PKCL, PRK CL, PRKCH, nPKC-eta
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 73-99 nmol/min·mg
• mol wt: ~73 kDa
• UniProt accession no.: P24723
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PRKCH(5583)

Description

General description

PKCη (protein kinase C η) belongs to the protein kiinase C family. It is mainly expressed in the squamous cell epithelia.[1]

Biochem/physiol Actions

PKCη (protein kinase C η) mainly participates in terminal differentiation of keratinocytes. It is responsible for arresting the cell cycle at G1 phase. It is also involved in phosphorylation of p21 and suppression of cdk2 (cyclin-dependent kinase 2) kinase activity by associating with cyclin E/cdk2/p21 complex, thereby regulating cell cycle.[1] PKCη is upregulated in breast cancer and is associated with cell growth.[2]

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)