K4377

Sigma-Aldrich

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse

clone KP-53, ascites fluid

Synonym(s):
Monoclonal Anti-Human Kappa Light Chain (Bound and Free)
MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

KP-53, monoclonal

contains

15 mM sodium azide

application(s)

indirect ELISA: 1:10,000

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.

Specificity

The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.

Application

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:
  • immunodetection
  • in western blot analysis
  • in intracellular kappa light chain staining for flow cytometric analysis

Physical form

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse is provided as ascites fluid with 0.1% sodium azide (see MSDS) as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37-60

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy
Mathias S, et al.
Journal of Biotechnology, 271, 47-55 (2018)
Upregulated expression of kappa light chain by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cells via NF-kappaB and AP-1 pathways
Liu H, et al.
Cellular Signalling, 19(2), 419-427 (2007)
Heterogeneity of aberrant immunoglobulin expression in cancer cells
Hu D, et al.
Cellular & Molecular Immunology, 8(6), 479-479 (2011)
Chloe L Stoyle et al.
The Biochemical journal, 474(18), 3179-3188 (2017-08-09)
Rodent monoclonal antibodies with specificity towards important biological targets are developed for therapeutic use by a process of humanisation. This process involves the creation of molecules, which retain the specificity of the rodent antibody but contain predominantly human coding sequence....
Expression and secretion of immunoglobulin alpha heavy chain with diverse VDJ recombinations by human epithelial cancer cells
Zheng H, et al.
Molecular Immunology, 44(9), 2221-2227 (2007)

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