KCQS00

KiCqStart^® SYBR^® Green qPCR ReadyMix^™

For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

• Synonym(s): qPCR master mix, sybr green qPCR
• NACRES: NA.55
• UNSPSC Code: 41106300

Properties

• form: liquid
• usage: sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
• feature: dNTPs included, hotstart
• storage condition: protect from light
• technique(s): RT-qPCR: suitable, qPCR: suitable
• color: colorless
• input: purified DNA
• compatibility: for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Bio-Rad MyiQ, for use with Eppendorf^® Mastercycler ep realplex2 s, for use with Eppendorf^® Mastercycler ep realplex, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480
• detection method: SYBR^® Green
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Application

PCR applications:

• Gene expression
• DNA quantification
• CHiP

KiCqStart^® SYBR^® Green qPCR ReadyMix^™ has been used to perform quantitative real-time PCR (qPCR) for the amplification and quantification of genomic DNA extracted from human gut bacterium Prevotella copri.[1]

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Other Notes

2X reaction buffer containing optimized concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Instrument Compatibility:
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:

• Bio-Rad^® CFX384^™
• Bio-Rad CFX96^™
• Bio-Rad MiniOpticon^™
• Bio-Rad/MJ Chromo4^™
• Bio-Rad/MJ Opticon 2
• Bio-Rad/MJ Opticon^™
• Cepheid SmartCycler^®
• Eppendorf Mastercycler^® ep realplex
• Eppendorf Mastercycler ep realplex2 s
• Illumina Eco qPCR
• Qiagen/Corbett Rotor-Gene^® 3000
• Qiagen/Corbett Rotor-Gene 6000
• Qiagen/Corbett Rotor-Gene Q
• Roche LightCycler^® 480

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

Eppendorf is a registered trademark of Eppendorf SE

KiCqStart is a registered trademark of QIAGEN Beverly Inc.

LightCycler is a registered trademark of Roche

Mastercycler is a registered trademark of Eppendorf SE

MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.

Opticon is a trademark of Bio-Rad Laboratories, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Rotor-Gene is a registered trademark of Qiagen GmbH

SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

SmartCycler is a registered trademark of Cepheid, Inc.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable