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KCQS03

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix

iQ, with fluorescein for Bio-Rad systems

NACRES:
NA.55

form

liquid

usage

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

feature

dNTPs included
hotstart

storage condition

protect from light

technique(s)

qPCR: suitable

color

colorless

input

purified DNA

compatibility

for use with Bio-Rad MyiQ
for use with Bio-Rad iCycler iQ
for use with Bio-Rad iQ 5

detection method

SYBR® Green

shipped in

dry ice

storage temp.

−20°C

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General description

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Application

KiCqStart® SYBR® Green qPCR ReadyMix has been used for the qPCR analysis of 16S rRNA.
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
PCR applications:
  • Gene expression
  • DNA quantification
  • CHiP

Features and Benefits

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Other Notes

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Legal Information

KiCqStart is a registered trademark of Qiagen Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iQ is a trademark of Bio-Rad Laboratories, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Himanshu Kumar Khuntia et al.
SN applied sciences, 2(8), 1320-1320 (2020-08-25)
This research aims to determine the presence of antibiotic-resistant genes (ARG) in anaerobic biofilm reactors (ABR) fed with household chemical products (HCP) such as laundry detergents and handwash without any influx of antibiotics. The ABR comprised a three-chamber design with
Jackson T Sparks et al.
Insect biochemistry and molecular biology, 43(12), 1161-1171 (2013-10-26)
The yellow-fever mosquito, Aedes aegypti, infects a growing number of people every year with dengue, yellow fever and chikungunya viruses. Contact chemoreception in mosquitoes influences a number of behaviors including host-selection, oviposition and feeding. While these behaviors are in many
Glade Dlott et al.
Journal of microbiological methods, 115, 112-120 (2015-06-10)
We tested a method of estimating the activity of detectable individual bacterial and archaeal OTUs within a community by calculating ratios of absolute 16S rRNA to rDNA copy numbers. We investigated phylogenetically coherent patterns of activity among soil prokaryotes in
Andreas Varkaris et al.
International journal of cancer, 133(7), 1536-1546 (2013-03-26)
The receptor tyrosine kinase, MET, has been implicated in tumorigenesis and metastasis of many solid tumors, by multiple mechanisms, including cross talk with epidermal growth factor receptor. In this study, we examined the role of insulin-like growth factor receptor-1 (IGF-1R)
Christine E Prasse et al.
Applied and environmental microbiology, 81(10), 3482-3491 (2015-03-15)
Restored wetland soils differ significantly in physical and chemical properties from their natural counterparts even when plant community compositions are similar, but effects of restoration on microbial community composition and function are not well understood. Here, we investigate plant-microbe relationships

Articles

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Protocols

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Primer Optimization Protocol Using Temperature Gradient

Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.

qPCR Efficiency Determination Protocol

Once an assay has been optimized, it is important to verify the reaction efficiency. This information is important when reporting and comparing assays. In this example protocol, the assay efficiency is compared over a wide and narrow dynamic range of cDNA concentrations.

qPCR Gene Expression/Copy Number Analysis Protocol Using SYBR Green I Dye Detection

Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be adapted to specific experimental needs.

See All

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SYBR® Green Based Quantitative PCR

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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