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KCQS05

Sigma-Aldrich

KiCqStart® Probe qPCR ReadyMix, Low ROX

with Low ROX for ABI and Stratagene instruments

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Synonym(s):
Kicqstart® Probe qPCR ReadyMix
NACRES:
NA.55

form

liquid

usage

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

feature

dNTPs included
hotstart

storage condition

protect from light

technique(s)

qPCR: suitable

color

light blue

input

purified DNA

compatibility

ABI 7500 Fast
ABI 7500
ABI QuantStudio
ABI ViiA 7
Agilent AriaMx
Douglas Scientific IntelliQube
Qiagen Corbett Rotor-Gene Q
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000

detection method

probe-based

shipped in

dry ice

storage temp.

−20°C

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KCQS06KCQS08KCQS09
form

liquid

form

liquid

form

liquid

form

liquid

usage

sufficient for 1250 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 500 reactions

usage

sufficient for 500 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

color

light blue

color

light blue

color

light blue

color

light blue

General description

KiCqStart® Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

KiCqStart® Probe qPCR ReadyMix, Low ROX has been used as a component of reaction mixture for detection of genomic DNA extracted from parasites by multiplex quantitative polymerase chain reaction (qPCR) and Real-Time PCR (qPCR).
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.

Features and Benefits

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

KiCqStart is a registered trademark of Qiagen Beverly Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Tamirat T Temesgen et al.
Foods (Basel, Switzerland), 10(2) (2021-03-07)
Due to the delicate nature of berries and the reduced shelf-life once washed, producers usually do not wash berries. Therefore, consumers are expected to wash the berries prior to consumption, and this might be a more effective way of infection
Surveillance of berries sold on the Norwegian market for parasite contamination using molecular methods
Temesgen TT, et al.
Food Microbiology, 103980-103980 (2022)
Alessandra Barlaam et al.
Food microbiology, 98, 103792-103792 (2021-04-21)
To investigate the presence of Cyclospora cayetanensis, Toxoplasma gondii and Echinococcus spp. in fresh produce sold in Italy, 324 locally produced 'ready-to-eat' (RTE) mixed-salad packages belonging to three brands and 324 berries packages (blueberries and blackberries imported from Peru and
Tamirat Tefera Temesgen et al.
Food research international (Ottawa, Ont.), 125, 108636-108636 (2019-09-27)
Foodborne parasites (FBP) are of major public health importance and warrant appropriate detection and control strategies. Most of the FBP considered for risk-ranking by a panel of experts are potentially transmitted via consumption of contaminated fresh produce, including berries. In

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PCR/qPCR Data Analysis

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Protocols

KiCqStart™ Probe qPCR ReadyMix™

KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service