Pre-designed RT-qPCR Primers, SYBR Green RT-qPCR Primers
Ordering predesigned KiCqStart SYBR® Green primers for your gene targets is easy using Sigma's state-of-the-art oligonucleotide configuration tool. Open the tool, enter your gene, select your species, choose your primer sets, and add to cart.
Overview Compatible with any thermal cycler, KiCqStart Primers are ready-to-order, pre-designed primer pairs for quantifying gene expression by SYBR Green I RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Primers are available as up to three ranked sets of forward and reverse primer pairs for all genes from common model organisms.
1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.