The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
L0543
LB Agar Kanamycin-50, Plates
pre-poured LB agar plates with kanamycin sulfate
Synonym(s):
kanamycin agar plates
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About This Item
UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
selective for Escherichia coli
selective for coliforms
selective for coliforms
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grade
Molecular Biology
sterility
sterile; autoclaved
form
plates (10 cm)
plates (10cm, 20-25ml each)
capacity
20-25 mL
storage temp.
2-8°C
suitability
selective for Escherichia coli
selective for coliforms
1 of 4
This Item | L5667 | L3147 | L2542 |
|---|---|---|---|
| sterility sterile; autoclaved | sterility sterile; autoclaved | sterility non-sterile | sterility sterile; 0.2 μm filtered |
| suitability selective for Escherichia coli, selective for coliforms | suitability selective for Escherichia coli, selective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| form plates (10 cm) | form plates (10 cm), plates (10cm, 20-25ml each) | form powder | form liquid |
| capacity 20-25 mL | capacity 20-25 mL | capacity - | capacity - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp | storage temp. room temp |
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens[1] and E.coli JM83 strain with pGD103 plasmid.[2]
Features and Benefits
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Components
15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Why do the LB-agar plates with antibiotics have such a short expiration date?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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