The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
L0543
LB Agar Kanamycin-50, Plates
pre-poured LB agar plates with kanamycin sulfate
Synonym(s):
kanamycin agar plates
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 ea | Check Cart for Availability | $54.20 |
About This Item
UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
selective for Escherichia coli, selective for coliforms
$54.20
Check Cart for Availability
grade
Molecular Biology
Quality Level
sterility
sterile; autoclaved
form
plates (10 cm), plates (10cm, 20-25ml each)
capacity
20-25 mL
storage temp.
2-8°C
suitability
selective for Escherichia coli, selective for coliforms
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens and E.coli JM83 strain with pGD103 plasmid.
Features and Benefits
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Other Notes
15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
1 of 1
This Item | |||
|---|---|---|---|
| sterility sterile; autoclaved | sterility sterile; autoclaved | sterility non-sterile | sterility sterile; 0.2 μm filtered |
| suitability selective for Escherichia coli, selective for coliforms | suitability selective for Escherichia coli, selective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form plates (10 cm), plates (10cm, 20-25ml each) | form plates (10 cm), plates (10cm, 20-25ml each) | form powder | form liquid |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp | storage temp. room temp |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles
Talhinhas P, et al.
Molecular Biotechnology, 39(1), 57-67 (2008)
Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae.
Deich RA, et al.
Journal of Bacteriology, 170(2), 489-498 (1988)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L0543-10EA | 04061833948828 |
-
Why do the LB-agar plates with antibiotics have such a short expiration date?
1 answer-
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-