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MilliporeSigma

L0543

Sigma-Aldrich

LB Agar Kanamycin-50, Plates

pre-poured LB agar plates with kanamycin sulfate

Synonym(s):

kanamycin agar plates

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About This Item

UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
selective for Escherichia coli
selective for coliforms

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grade

Molecular Biology

sterility

sterile; autoclaved

form

plates (10 cm)
plates (10cm, 20-25ml each)

capacity

20-25 mL

storage temp.

2-8°C

suitability

selective for Escherichia coli
selective for coliforms

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1 of 4

This Item
L5667L3147L2542
sterility

sterile; autoclaved

sterility

sterile; autoclaved

sterility

non-sterile

sterility

sterile; 0.2 μm filtered

suitability

selective for Escherichia coli, selective for coliforms

suitability

selective for Escherichia coli, selective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

form

plates (10 cm)

form

plates (10 cm), plates (10cm, 20-25ml each)

form

powder

form

liquid

capacity

20-25 mL

capacity

20-25 mL

capacity

-

capacity

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens[1] and E.coli JM83 strain with pGD103 plasmid.[2]

Features and Benefits

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Components

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

1–2 of 2 Questions  
  1. Why do the LB-agar plates with antibiotics have such a short expiration date?

    1 answer
    1. The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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