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L0663

Sigma-Aldrich

Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal

clone 4H8-2, purified from hybridoma cell culture

Synonym(s):
Anti-Merosin
MDL number:
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4H8-2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~400 kDa (denatured)

species reactivity

human, mouse

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 4-8 μg/mL using acetone-fixed frozen sections of human tongue
microarray: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... LAMA2(3908)
mouse ... Lama2(16773)

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L9393MABT39SAB5700147
biological source

rat

biological source

rabbit

biological source

mouse

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

clone

4H8-2, monoclonal

clone

polyclonal

clone

4B12, monoclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

mol wt

antigen ~400 kDa (denatured)

mol wt

-

mol wt

-

mol wt

66

General description

The gene LAMA2 (laminin-2) encodes a protein belonging to a large family of heterotrimeric glycoproteins called laminins. It contains a large globular C-terminal domain that binds to dystroglycan and integrin α7β1 cell receptor specific for myofibers. The amino terminal domain associates with other laminins during the formation of protein meshwork that is involved in the basal membrane supramolecular assembly. The gene is mapped to human chromosome 6q22.33.
Laminin, the most abundant structural and biologically active component in basement membrane, is a complex extracellular glycoprotein of 700-900 kDa that plays an important role in many aspects of the cell biology. It is composed of one α chain (approx. 400 kDa, previously called A chain) one β chain (215 kDa, B1) and one γ chain (205 kDa, B2), held together by disulfide bonds.
Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal (rat IgG1 isotype) is derived from the 4H8-2 hybridoma produced by the fusion of rat myeloma cells and splenocytes from a Lewis rat immunized with mouse heart laminin-2.1 The antibody is purified from the culture supernatant of hybridoma cells grown in a bioreactor. Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal reacts specifically with mouse1 and human2, laminin-2. The epitope recognized by the antibody resides in the N-terminal portion of the α2 chain of laminin.

Specificity

The epitope recognized by the antibody resides in the N-terminal portion of the α2 chain of laminin.

Immunogen

Mouse heart laminin-2

Application

Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal has been used in immunofluorescence and immunohistochemistry.

Biochem/physiol Actions

Laminins are glycoproteins that function in extracellular matrix architecture, cell adhesion, differentiation, and neurite outgrowth. Mutations in the gene LAMA2 (laminin-2) leading to either partial deficiency or complete absence of laminin α2 have been associated with limb-girdle muscular dystrophy (LGMD), characterized by severe, early-onset congenital muscular dystrophy to mild, later childhood-onset limb-girdle type muscular dystrophy, hypotonia with muscle weakness at early infancy, poor spontaneous movements with contractures of the large joints, and weak cry along with respiratory dysfunction.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from the culture supernatant of hybridoma cells grown in a bioreactor.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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