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L1418

Sigma-Aldrich

Anti-LAMP1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
LAMP1 Antibody - Anti-LAMP1 antibody produced in rabbit, Anti-CD107a, Anti-LAMPA, Lamp1 Antibody, Anti-LGP120, Anti-Lysosomal-associated membrane protein 1
MDL number:

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~120 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HeLa, rat NRK, and mouse NIH3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... LAMP1(3916)
mouse ... Lamp1(16783)
rat ... Lamp1(25328)

General description

The gene LAMP1 (lysosomal-associated membrane protein 1) encodes a type I transmembrane protein that has a short cytoplasmic tail containing a lysosome-targeting signal of GYQTI(382)-COOH. The gene is mapped to human chromosome 13q34.
Lysosome-associated membrane protein 1 (LAMP1), also termed Igp120, is a heavily glycosylated lysosomal membrane protein of about 120 kDa. It consists of a ~40 kDa core polypeptide with O-linked and 18 asparagine-linked oligosaccharide side chains. LAMP1 protein contains a leader sequence, a large intralumenal region consisting of 2 homologous domains separated by a hinge region rich in proline and serine, a 24-amino acid transmembrane region, and a short cytoplasmic tail containing the lysosomal membrane targeting signal. LAMP1 is ubiquitously expressed and highly conserved. It localizes mainly to lysosomes although a small portion is detected on the cell surface. It was found that highly metastatic tumor cells express more LAMP molecules on the cell surface than poorly metastatic cells.

Immunogen

synthetic peptide corresponding to amino acid residues 405-416 of human LAMP1 with N-terminal added cysteine-glycine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Anti-LAMP1-Cy3® antibody produced in rabbit has been used in western blotting and immunofluorescence.

Biochem/physiol Actions

The gene LAMP1 (lysosomal associated membrane protein 1) encodes a membrane glycoprotein that functions as an intracellular receptor. It is found to be expressed in the cytoplasm of several types of tumor cells and may be involved in tumor invasion. Lamp1 is crucial for perforin trafficking to the lytic granules and motility of these lytic granules. Its knockdown leads to inhibition of cytotoxicity of human natural killer cells.
Lysosome-associated membrane protein 1 (LAMP1) with heavy glycosylation, may be important to protect the lysosomal membrane from proteolytic enzymes within lysosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

CAB-O-SIL is a registered trademark of Cabot Corp.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Tong Wang et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(15), 6179-6194 (2015-04-17)
Botulinum neurotoxin type A (BoNT/A) is a highly potent neurotoxin that elicits flaccid paralysis by enzymatic cleavage of the exocytic machinery component SNAP25 in motor nerve terminals. However, recent evidence suggests that the neurotoxic activity of BoNT/A is not restricted
Down-regulation of alphav/beta3 integrin via misrouting to lysosomes by overexpression of a beta3Lamp1 fusion protein
Conesa M, et al.
The Biochemical Journal, 370(2), 703-711 (2003)
Saponins modulate the intracellular trafficking of protein toxins.
Weng A
Journal of Controlled Release : Official Journal of the Controlled Release Society, 164, 74-86 (2012)
Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking.
Fukuda M
Test, 266(32), 21327-21330 (1991)
Stephen DiGiuseppe et al.
Journal of virology, 89(20), 10442-10452 (2015-08-08)
The human papillomavirus (HPV) capsid is composed of the major capsid protein L1 and the minor capsid protein L2. During entry, the HPV capsid undergoes numerous conformational changes that result in endosomal uptake and subsequent trafficking of the L2 protein

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