L1668

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

• Synonym(s): Phaseolus vulgaris agglutinin, PHA
• NACRES: NA.32

Properties

• Quality Level: 200
• product line: BioReagent
• form: lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)
• potency: ≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)
• technique(s): cell culture | mammalian: suitable
• color: white to off-white
• solubility: PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)
• storage temp.: 2-8°C

Description

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:

• for the stimulation of cluster of differentiation (CD4+) T cells[1]
• for the activation of primary peripheral blood lymphocytes[2]
• for the stimulation of peripheral blood mononuclear cells[3][4]

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

Sold on the basis of lectin content

Analysis Note

Potency and solubility expressed in mg lectin/ml

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves