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L1882

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

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Synonym(s):
WGA, Wheat germ agglutinin
NACRES:
NA.32

conjugate

agarose conjugate

Quality Level

form

saline suspension

extent of labeling

5-10 mg per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

capacity

≥0.1 mg/mL binding capacity (ovomucoid)

storage temp.

2-8°C

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This Item
L1394L2895T4144
form

saline suspension

form

saline suspension

form

lyophilized powder

form

lyophilized powder

matrix

cross-linked 4% beaded agarose

matrix

4% agarose

matrix

-

matrix

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

extent of labeling

5-10 mg per mL

extent of labeling

~6 mg per mL

extent of labeling

≥1 mol FITC per mol lectin

extent of labeling

-

General description

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.

Biochem/physiol Actions

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Physical form

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Preparation Note

Conjugates are prepared from affinity purified lectin.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Articles

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

This page shows how to prepare samples for purification with affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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